CEACAMs interaction with Helicobacter pylori HopQ supports the type 4 secretion system-dependent activation of non-canonical NF-κB
Helicobacter pylori infection represents a major risk factor for the development of gastric diseases and gastric cancer. The capability of H. pylori to inject the virulence factor cytotoxin-associated gene A (CagA) depends on a type IV secretion system (T4SS) encoded by the cag pathogenicity island...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-09-01
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| Series: | International Journal of Medical Microbiology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1438422120300540 |
| _version_ | 1798032787378274304 |
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| author | Gunter Maubach Olga Sokolova Christian Täger Michael Naumann |
| author_facet | Gunter Maubach Olga Sokolova Christian Täger Michael Naumann |
| author_sort | Gunter Maubach |
| collection | DOAJ |
| description | Helicobacter pylori infection represents a major risk factor for the development of gastric diseases and gastric cancer. The capability of H. pylori to inject the virulence factor cytotoxin-associated gene A (CagA) depends on a type IV secretion system (T4SS) encoded by the cag pathogenicity island (cagPAI). Further, infection by H. pylori activates the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) in a T4SS-dependent manner but CagA-independent manner. Here we investigated the role of host cell receptors carcinoembryonic antigen-related cell adhesion molecules (CEACAMs) and the bacterial adhesin HopQ in the activation of non-canonical NF-κB and CagA translocation into gastric epithelial cells. AGS cells express six of twelve CEACAMs found in humans. In HeLa cells, only CEACAM19 is expressed. We showed that deletion of hopQ attenuates the activation of non-canonical NF-κB only in AGS but not in HeLa cells. CagA translocation was in both cell lines affected by HopQ depletion, although to a much lesser extent in HeLa cells. Moreover, we observed a possible redundancy between the three HopQ-binding CEACAMs 1, 5 and 6 and their capacity to support non-canonical NF-κB activation. Our results illustrate that the interaction between HopQ and CEACAMs could promote the efficiency of the T4SS. |
| first_indexed | 2024-04-11T20:19:35Z |
| format | Article |
| id | doaj.art-a419bdd511544a6eacfce4fdf205ca78 |
| institution | Directory Open Access Journal |
| issn | 1438-4221 |
| language | English |
| last_indexed | 2024-04-11T20:19:35Z |
| publishDate | 2020-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal of Medical Microbiology |
| spelling | doaj.art-a419bdd511544a6eacfce4fdf205ca782022-12-22T04:04:52ZengElsevierInternational Journal of Medical Microbiology1438-42212020-09-013106151444CEACAMs interaction with Helicobacter pylori HopQ supports the type 4 secretion system-dependent activation of non-canonical NF-κBGunter Maubach0Olga Sokolova1Christian Täger2Michael Naumann3Otto von Guericke University, Institute of Experimental Internal Medicine, Medical Faculty, Leipziger Str. 44, 39120, Magdeburg, GermanyOtto von Guericke University, Institute of Experimental Internal Medicine, Medical Faculty, Leipziger Str. 44, 39120, Magdeburg, GermanyOtto von Guericke University, Institute of Experimental Internal Medicine, Medical Faculty, Leipziger Str. 44, 39120, Magdeburg, GermanyCorresponding author.; Otto von Guericke University, Institute of Experimental Internal Medicine, Medical Faculty, Leipziger Str. 44, 39120, Magdeburg, GermanyHelicobacter pylori infection represents a major risk factor for the development of gastric diseases and gastric cancer. The capability of H. pylori to inject the virulence factor cytotoxin-associated gene A (CagA) depends on a type IV secretion system (T4SS) encoded by the cag pathogenicity island (cagPAI). Further, infection by H. pylori activates the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) in a T4SS-dependent manner but CagA-independent manner. Here we investigated the role of host cell receptors carcinoembryonic antigen-related cell adhesion molecules (CEACAMs) and the bacterial adhesin HopQ in the activation of non-canonical NF-κB and CagA translocation into gastric epithelial cells. AGS cells express six of twelve CEACAMs found in humans. In HeLa cells, only CEACAM19 is expressed. We showed that deletion of hopQ attenuates the activation of non-canonical NF-κB only in AGS but not in HeLa cells. CagA translocation was in both cell lines affected by HopQ depletion, although to a much lesser extent in HeLa cells. Moreover, we observed a possible redundancy between the three HopQ-binding CEACAMs 1, 5 and 6 and their capacity to support non-canonical NF-κB activation. Our results illustrate that the interaction between HopQ and CEACAMs could promote the efficiency of the T4SS.http://www.sciencedirect.com/science/article/pii/S1438422120300540Cytotoxin-associated gene A (CagA)NF-κB-inducing kinase (NIK)Outer membrane protein (OMP)p100RelARelB |
| spellingShingle | Gunter Maubach Olga Sokolova Christian Täger Michael Naumann CEACAMs interaction with Helicobacter pylori HopQ supports the type 4 secretion system-dependent activation of non-canonical NF-κB International Journal of Medical Microbiology Cytotoxin-associated gene A (CagA) NF-κB-inducing kinase (NIK) Outer membrane protein (OMP) p100 RelA RelB |
| title | CEACAMs interaction with Helicobacter pylori HopQ supports the type 4 secretion system-dependent activation of non-canonical NF-κB |
| title_full | CEACAMs interaction with Helicobacter pylori HopQ supports the type 4 secretion system-dependent activation of non-canonical NF-κB |
| title_fullStr | CEACAMs interaction with Helicobacter pylori HopQ supports the type 4 secretion system-dependent activation of non-canonical NF-κB |
| title_full_unstemmed | CEACAMs interaction with Helicobacter pylori HopQ supports the type 4 secretion system-dependent activation of non-canonical NF-κB |
| title_short | CEACAMs interaction with Helicobacter pylori HopQ supports the type 4 secretion system-dependent activation of non-canonical NF-κB |
| title_sort | ceacams interaction with helicobacter pylori hopq supports the type 4 secretion system dependent activation of non canonical nf κb |
| topic | Cytotoxin-associated gene A (CagA) NF-κB-inducing kinase (NIK) Outer membrane protein (OMP) p100 RelA RelB |
| url | http://www.sciencedirect.com/science/article/pii/S1438422120300540 |
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