Protocol for performing pooled CRISPR-Cas9 loss-of-function screens

Protocol for performing pooled CRISPR-Cas9 loss-of-function screens

Summary: Phenotypic screens involving pooled CRISPR-Cas9 libraries offer a powerful, rapid yet affordable approach to evaluate gene functions on a global scale. Here, we present a protocol for performing pooled CRISPR-Cas9 loss-of-function screens to identify genetic modifiers using either fluoresce...

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Bibliographic Details
Main Authors: Alyssa J. Mathiowetz, Melissa A. Roberts, David W. Morgens, James A. Olzmann, Zhipeng Li
Format: Article
Language:English
Published: Elsevier 2023-06-01
Series:STAR Protocols
Subjects:
Cell Culture
Flow Cytometry/Mass Cytometry
Cell-based Assays
Genomics
Molecular Biology
CRISPR
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723001594

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http://www.sciencedirect.com/science/article/pii/S2666166723001594

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