Protocol for isolation and spectral flow cytometry analysis of immune cells from the murine exocrine and endocrine pancreas

Summary: Diabetes mellitus is a disease of the hormone-secreting endocrine pancreas. However, increasing evidence suggests that the exocrine pancreas is also involved in the pathogenesis of diabetes. In this protocol, we describe how to harvest both isolated islets and exocrine tissue from one mouse...

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Bibliographic Details
Main Authors: Laura Steiger, Zora Baumann, Lena Keller, Marianne Böni-Schnetzler, Marc Y. Donath, Daniel T. Meier
Format: Article
Language:English
Published: Elsevier 2023-12-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723006317