Quantifying 35 transcripts in a single tube: model-based calibration of the GeXP multiplex RT-PCR assay

Quantifying 35 transcripts in a single tube: model-based calibration of the GeXP multiplex RT-PCR assay

Abstract Background Quantitative analysis of differential gene expression is of central importance in molecular life sciences. The Gene eXpression Profiling technology (GeXP) relies on multiplex RT-PCR and subsequent capillary electrophoretic separation of the amplification products and allows to qu...

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Bibliographic Details
Main Authors: Pauline Marquardt, Britta Werthmann, Viktoria Rätzel, Markus Haas, Wolfgang Marwan
Format: Article
Language:English
Published: BMC 2021-04-01
Series:BMC Biotechnology
Subjects:
Multiplex RT-PCR
DNA fragment analysis
Capillary electrophoresis
Cell differentiation
Marker genes
Physarum polycephalum
Online Access:https://doi.org/10.1186/s12896-021-00689-4

Internet

https://doi.org/10.1186/s12896-021-00689-4

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