A computational approach to analyze the missense mutations in human angiogenin variants leading to amyotrophic lateral sclerosis
The most detrimental missense mutations of angiogenin causing Amyotrophic lateral sclerosis were identified computationally and the substrate binding efficiencies of these mutations were also analyzed. Out of 12 variants, I-Mutant 2.0, SIFT and PolyPhen programs identified 3 variants that were less...
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| Format: | Article |
| Language: | English |
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Bangladesh Pharmacological Society
2013-11-01
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| Series: | Bangladesh Journal of Pharmacology |
| Subjects: | |
| Online Access: | https://www.banglajol.info/index.php/BJP/article/view/15259 |
| _version_ | 1819044455682932736 |
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| author | K. Sreevishnupriya Ramalingar Rajasekaran |
| author_facet | K. Sreevishnupriya Ramalingar Rajasekaran |
| author_sort | K. Sreevishnupriya |
| collection | DOAJ |
| description | The most detrimental missense mutations of angiogenin causing Amyotrophic lateral sclerosis were identified computationally and the substrate binding efficiencies of these mutations were also analyzed. Out of 12 variants, I-Mutant 2.0, SIFT and PolyPhen programs identified 3 variants that were less stable, deleterious and damaging respectively. Modeling of these 3 variants was performed to understand the change in their conformations with respect to the native angiogenin by computing their RMSD and Total energy. The native and the 3 mutants were docked with ribonuclease inhibitor to explain the binding efficiencies of those detrimental missense mutations. The loss of binding affinity with their interacting protein namely ribonuclease inhibitor was investigated by computing the flexibility of binding amino acids of angiogenin and computing the binding free energy (ΔG) between native and mutant complexes. |
| first_indexed | 2024-12-21T10:12:57Z |
| format | Article |
| id | doaj.art-a4d4a870f2be424fb701bceedf6ff48b |
| institution | Directory Open Access Journal |
| issn | 1991-0088 |
| language | English |
| last_indexed | 2024-12-21T10:12:57Z |
| publishDate | 2013-11-01 |
| publisher | Bangladesh Pharmacological Society |
| record_format | Article |
| series | Bangladesh Journal of Pharmacology |
| spelling | doaj.art-a4d4a870f2be424fb701bceedf6ff48b2022-12-21T19:07:39ZengBangladesh Pharmacological SocietyBangladesh Journal of Pharmacology1991-00882013-11-018410.3329/bjp.v8i4.15259A computational approach to analyze the missense mutations in human angiogenin variants leading to amyotrophic lateral sclerosisK. Sreevishnupriya0Ramalingar Rajasekaran1Bioinformatics Division, School of Biosciences and Technology, Vellore Institute of Technology, Vellore 632014, Tamil NaduBioinformatics Division, School of Biosciences and Technology, Vellore Institute of Technology, Vellore 632014, Tamil NaduThe most detrimental missense mutations of angiogenin causing Amyotrophic lateral sclerosis were identified computationally and the substrate binding efficiencies of these mutations were also analyzed. Out of 12 variants, I-Mutant 2.0, SIFT and PolyPhen programs identified 3 variants that were less stable, deleterious and damaging respectively. Modeling of these 3 variants was performed to understand the change in their conformations with respect to the native angiogenin by computing their RMSD and Total energy. The native and the 3 mutants were docked with ribonuclease inhibitor to explain the binding efficiencies of those detrimental missense mutations. The loss of binding affinity with their interacting protein namely ribonuclease inhibitor was investigated by computing the flexibility of binding amino acids of angiogenin and computing the binding free energy (ΔG) between native and mutant complexes.https://www.banglajol.info/index.php/BJP/article/view/15259Amyotrophic lateral sclerosisAngiogeninFlexibilityFree energyMissense mutationProtein docking |
| spellingShingle | K. Sreevishnupriya Ramalingar Rajasekaran A computational approach to analyze the missense mutations in human angiogenin variants leading to amyotrophic lateral sclerosis Bangladesh Journal of Pharmacology Amyotrophic lateral sclerosis Angiogenin Flexibility Free energy Missense mutation Protein docking |
| title | A computational approach to analyze the missense mutations in human angiogenin variants leading to amyotrophic lateral sclerosis |
| title_full | A computational approach to analyze the missense mutations in human angiogenin variants leading to amyotrophic lateral sclerosis |
| title_fullStr | A computational approach to analyze the missense mutations in human angiogenin variants leading to amyotrophic lateral sclerosis |
| title_full_unstemmed | A computational approach to analyze the missense mutations in human angiogenin variants leading to amyotrophic lateral sclerosis |
| title_short | A computational approach to analyze the missense mutations in human angiogenin variants leading to amyotrophic lateral sclerosis |
| title_sort | computational approach to analyze the missense mutations in human angiogenin variants leading to amyotrophic lateral sclerosis |
| topic | Amyotrophic lateral sclerosis Angiogenin Flexibility Free energy Missense mutation Protein docking |
| url | https://www.banglajol.info/index.php/BJP/article/view/15259 |
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