Isolation and Antibiotic Resistant Research of <i>Tetragenococcus halophilus</i> from Xuanwei Ham, A China High-Salt-Fermented Meat Products
We assessed the prevalence of antibiotic resistant and antibiotic resistance genes for 49 <i>Tetragenococcus halophilus</i> (<i>T. halophilus</i>) strains isolated from Xuawei ham in China. The antibiotic resistance phenotype was detected by the Bauer−Kirby (K&#...
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MDPI AG
2019-09-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/8/3/151 |
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| author | Yinjiao Li Luying Shan Chen Zhang Zhan Lei Ying Shang |
| author_facet | Yinjiao Li Luying Shan Chen Zhang Zhan Lei Ying Shang |
| author_sort | Yinjiao Li |
| collection | DOAJ |
| description | We assessed the prevalence of antibiotic resistant and antibiotic resistance genes for 49 <i>Tetragenococcus halophilus</i> (<i>T. halophilus</i>) strains isolated from Xuawei ham in China. The antibiotic resistance phenotype was detected by the Bauer−Kirby (K−B) method and the results showed that 49 isolates can be considered completely susceptible to penicillin, ampicillin, amoxicillin, cefradine, cefotaxime, tetracyclines, minocycline, doxycycline, and vancomycin, but resistant to gentamicin, streptomycin, neomycin, polymyxinB, cotrimoxazole. This resistance was sufficiently high to consider the potential for acquisition of transmissible determinants. A total of 32 isolates were resistant to ofloxacin, 4 isolates were resistant to ciprofloxacin and chloramphenicol, and 2 isolates were resistant to ceftazidime and ticarcillin. The antibiotic resistance genes were detected by routine polymerase chain reaction (PCR). Among the 26 antibiotic resistance genes, 5 varieties of antibiotic resistance genes, including <i>acrB</i>, <i>blaTEM</i>, <i>AAda1</i>, <i>SulII</i>, and <i>GyrB</i> were detected and the detection rates were 89.79%, 47.7%, 16.33%, 77.55%, and 75.51%, respectively. The potential acquisition of transmissible determinants for antibiotic resistance and antibiotic resistance genes identified in this study necessitate the need for a thorough antibiotic resistance safety assessment of <i>T. halophilus</i> before it can be considered for use in food fermentation processes. |
| first_indexed | 2024-12-22T13:18:11Z |
| format | Article |
| id | doaj.art-a4ec812a2a1b49b0b22477d9a8694d15 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-12-22T13:18:11Z |
| publishDate | 2019-09-01 |
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| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-a4ec812a2a1b49b0b22477d9a8694d152022-12-21T18:24:32ZengMDPI AGAntibiotics2079-63822019-09-018315110.3390/antibiotics8030151antibiotics8030151Isolation and Antibiotic Resistant Research of <i>Tetragenococcus halophilus</i> from Xuanwei Ham, A China High-Salt-Fermented Meat ProductsYinjiao Li0Luying Shan1Chen Zhang2Zhan Lei3Ying Shang4Yunnan Institute of Food Safety, Kunming University of Science and Technology, Yunnan 650500, ChinaYunnan Institute of Food Safety, Kunming University of Science and Technology, Yunnan 650500, ChinaYunnan Institute of Food Safety, Kunming University of Science and Technology, Yunnan 650500, ChinaYunnan Institute of Food Safety, Kunming University of Science and Technology, Yunnan 650500, ChinaYunnan Institute of Food Safety, Kunming University of Science and Technology, Yunnan 650500, ChinaWe assessed the prevalence of antibiotic resistant and antibiotic resistance genes for 49 <i>Tetragenococcus halophilus</i> (<i>T. halophilus</i>) strains isolated from Xuawei ham in China. The antibiotic resistance phenotype was detected by the Bauer−Kirby (K−B) method and the results showed that 49 isolates can be considered completely susceptible to penicillin, ampicillin, amoxicillin, cefradine, cefotaxime, tetracyclines, minocycline, doxycycline, and vancomycin, but resistant to gentamicin, streptomycin, neomycin, polymyxinB, cotrimoxazole. This resistance was sufficiently high to consider the potential for acquisition of transmissible determinants. A total of 32 isolates were resistant to ofloxacin, 4 isolates were resistant to ciprofloxacin and chloramphenicol, and 2 isolates were resistant to ceftazidime and ticarcillin. The antibiotic resistance genes were detected by routine polymerase chain reaction (PCR). Among the 26 antibiotic resistance genes, 5 varieties of antibiotic resistance genes, including <i>acrB</i>, <i>blaTEM</i>, <i>AAda1</i>, <i>SulII</i>, and <i>GyrB</i> were detected and the detection rates were 89.79%, 47.7%, 16.33%, 77.55%, and 75.51%, respectively. The potential acquisition of transmissible determinants for antibiotic resistance and antibiotic resistance genes identified in this study necessitate the need for a thorough antibiotic resistance safety assessment of <i>T. halophilus</i> before it can be considered for use in food fermentation processes.https://www.mdpi.com/2079-6382/8/3/151Xuanwei ham<i>Tetragenococcus halophilus</i>antibiotic resistantantibiotic resistance genes |
| spellingShingle | Yinjiao Li Luying Shan Chen Zhang Zhan Lei Ying Shang Isolation and Antibiotic Resistant Research of <i>Tetragenococcus halophilus</i> from Xuanwei Ham, A China High-Salt-Fermented Meat Products Antibiotics Xuanwei ham <i>Tetragenococcus halophilus</i> antibiotic resistant antibiotic resistance genes |
| title | Isolation and Antibiotic Resistant Research of <i>Tetragenococcus halophilus</i> from Xuanwei Ham, A China High-Salt-Fermented Meat Products |
| title_full | Isolation and Antibiotic Resistant Research of <i>Tetragenococcus halophilus</i> from Xuanwei Ham, A China High-Salt-Fermented Meat Products |
| title_fullStr | Isolation and Antibiotic Resistant Research of <i>Tetragenococcus halophilus</i> from Xuanwei Ham, A China High-Salt-Fermented Meat Products |
| title_full_unstemmed | Isolation and Antibiotic Resistant Research of <i>Tetragenococcus halophilus</i> from Xuanwei Ham, A China High-Salt-Fermented Meat Products |
| title_short | Isolation and Antibiotic Resistant Research of <i>Tetragenococcus halophilus</i> from Xuanwei Ham, A China High-Salt-Fermented Meat Products |
| title_sort | isolation and antibiotic resistant research of i tetragenococcus halophilus i from xuanwei ham a china high salt fermented meat products |
| topic | Xuanwei ham <i>Tetragenococcus halophilus</i> antibiotic resistant antibiotic resistance genes |
| url | https://www.mdpi.com/2079-6382/8/3/151 |
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