Identification of cell barcodes from long-read single-cell RNA-seq with BLAZE
Abstract Long-read single-cell RNA sequencing (scRNA-seq) enables the quantification of RNA isoforms in individual cells. However, long-read scRNA-seq using the Oxford Nanopore platform has largely relied upon matched short-read data to identify cell barcodes. We introduce BLAZE, which accurately an...
Main Authors: | , , , , , , |
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Format: | Article |
Language: | English |
Published: |
BMC
2023-04-01
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Series: | Genome Biology |
Online Access: | https://doi.org/10.1186/s13059-023-02907-y |