A single-cell atlas of in vitro multiculture systems uncovers the in vivo lineage trajectory and cell state in the human lung
Abstract We present an in-depth single-cell atlas of in vitro multiculture systems on human primary airway epithelium derived from normal and diseased lungs of 27 individual donors. Our large-scale single-cell profiling identified new cell states and differentiation trajectories of rare airway epith...
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| Format: | Article |
| Language: | English |
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Nature Publishing Group
2023-08-01
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| Series: | Experimental and Molecular Medicine |
| Online Access: | https://doi.org/10.1038/s12276-023-01076-z |
| _version_ | 1797699529488728064 |
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| author | Woochan Lee Seyoon Lee Jung-Ki Yoon Dakyung Lee Yuri Kim Yeon Bi Han Rokhyun Kim Sungji Moon Young Jun Park Kyunghyuk Park Bukyoung Cha Jaeyong Choi Juhyun Kim Na-young Ha Kwhanmien Kim Sukki Cho Nam-Hyuk Cho Tushar J. Desai Jin-Haeng Chung Joo-Hyeon Lee Jong-Il Kim |
| author_facet | Woochan Lee Seyoon Lee Jung-Ki Yoon Dakyung Lee Yuri Kim Yeon Bi Han Rokhyun Kim Sungji Moon Young Jun Park Kyunghyuk Park Bukyoung Cha Jaeyong Choi Juhyun Kim Na-young Ha Kwhanmien Kim Sukki Cho Nam-Hyuk Cho Tushar J. Desai Jin-Haeng Chung Joo-Hyeon Lee Jong-Il Kim |
| author_sort | Woochan Lee |
| collection | DOAJ |
| description | Abstract We present an in-depth single-cell atlas of in vitro multiculture systems on human primary airway epithelium derived from normal and diseased lungs of 27 individual donors. Our large-scale single-cell profiling identified new cell states and differentiation trajectories of rare airway epithelial cell types in human distal lungs. By integrating single-cell datasets of human lung tissues, we discovered immune-primed subsets enriched in lungs and organoids derived from patients with chronic respiratory disease. To demonstrate the full potential of our platform, we further illustrate transcriptomic responses to various respiratory virus infections in vitro airway models. Our work constitutes a single-cell roadmap for the cellular and molecular characteristics of human primary lung cells in vitro and their relevance to human tissues in vivo. |
| first_indexed | 2024-03-12T04:10:08Z |
| format | Article |
| id | doaj.art-a5124a68abf946d1a4a08b7e81df62ac |
| institution | Directory Open Access Journal |
| issn | 2092-6413 |
| language | English |
| last_indexed | 2024-03-12T04:10:08Z |
| publishDate | 2023-08-01 |
| publisher | Nature Publishing Group |
| record_format | Article |
| series | Experimental and Molecular Medicine |
| spelling | doaj.art-a5124a68abf946d1a4a08b7e81df62ac2023-09-03T11:07:13ZengNature Publishing GroupExperimental and Molecular Medicine2092-64132023-08-015581831184210.1038/s12276-023-01076-zA single-cell atlas of in vitro multiculture systems uncovers the in vivo lineage trajectory and cell state in the human lungWoochan Lee0Seyoon Lee1Jung-Ki Yoon2Dakyung Lee3Yuri Kim4Yeon Bi Han5Rokhyun Kim6Sungji Moon7Young Jun Park8Kyunghyuk Park9Bukyoung Cha10Jaeyong Choi11Juhyun Kim12Na-young Ha13Kwhanmien Kim14Sukki Cho15Nam-Hyuk Cho16Tushar J. Desai17Jin-Haeng Chung18Joo-Hyeon Lee19Jong-Il Kim20Department of Biomedical Sciences, Seoul National University College of MedicineDepartment of Biomedical Sciences, Seoul National University College of MedicineDivision of Pulmonary, Allergy and Critical Care, Department of Medicine, Stanford University School of MedicineDepartment of Biomedical Sciences, Seoul National University College of MedicineInstitute of Endemic Diseases, Medical Research Center, Seoul National UniversityDepartment of Pathology and Translational Medicine, Seoul National University Bundang HospitalDepartment of Biomedical Sciences, Seoul National University College of MedicineInterdisciplinary Program in Cancer Biology, College of Medicine, Seoul National UniversityDepartment of Translational Medicine, Seoul National University College of MedicineGenomic Medicine Institute (GMI), Medical Research Center, Seoul National UniversityGenomic Medicine Institute (GMI), Medical Research Center, Seoul National UniversityDepartment of Biomedical Sciences, Seoul National University College of MedicineDepartment of Biomedical Sciences, Seoul National University College of MedicineInstitute of Endemic Diseases, Medical Research Center, Seoul National UniversityDepartment of Thoracic and Cardiovascular Surgery, Seoul National University Bundang HospitalDepartment of Thoracic and Cardiovascular Surgery, Seoul National University Bundang HospitalDepartment of Biomedical Sciences, Seoul National University College of MedicineDivision of Pulmonary, Allergy and Critical Care, Department of Medicine, Stanford University School of MedicineDepartment of Pathology and Translational Medicine, Seoul National University Bundang HospitalWellcome–MRC Cambridge Stem Cell Institute, Jeffrey Cheah Biomedical Centre, University of CambridgeDepartment of Biomedical Sciences, Seoul National University College of MedicineAbstract We present an in-depth single-cell atlas of in vitro multiculture systems on human primary airway epithelium derived from normal and diseased lungs of 27 individual donors. Our large-scale single-cell profiling identified new cell states and differentiation trajectories of rare airway epithelial cell types in human distal lungs. By integrating single-cell datasets of human lung tissues, we discovered immune-primed subsets enriched in lungs and organoids derived from patients with chronic respiratory disease. To demonstrate the full potential of our platform, we further illustrate transcriptomic responses to various respiratory virus infections in vitro airway models. Our work constitutes a single-cell roadmap for the cellular and molecular characteristics of human primary lung cells in vitro and their relevance to human tissues in vivo.https://doi.org/10.1038/s12276-023-01076-z |
| spellingShingle | Woochan Lee Seyoon Lee Jung-Ki Yoon Dakyung Lee Yuri Kim Yeon Bi Han Rokhyun Kim Sungji Moon Young Jun Park Kyunghyuk Park Bukyoung Cha Jaeyong Choi Juhyun Kim Na-young Ha Kwhanmien Kim Sukki Cho Nam-Hyuk Cho Tushar J. Desai Jin-Haeng Chung Joo-Hyeon Lee Jong-Il Kim A single-cell atlas of in vitro multiculture systems uncovers the in vivo lineage trajectory and cell state in the human lung Experimental and Molecular Medicine |
| title | A single-cell atlas of in vitro multiculture systems uncovers the in vivo lineage trajectory and cell state in the human lung |
| title_full | A single-cell atlas of in vitro multiculture systems uncovers the in vivo lineage trajectory and cell state in the human lung |
| title_fullStr | A single-cell atlas of in vitro multiculture systems uncovers the in vivo lineage trajectory and cell state in the human lung |
| title_full_unstemmed | A single-cell atlas of in vitro multiculture systems uncovers the in vivo lineage trajectory and cell state in the human lung |
| title_short | A single-cell atlas of in vitro multiculture systems uncovers the in vivo lineage trajectory and cell state in the human lung |
| title_sort | single cell atlas of in vitro multiculture systems uncovers the in vivo lineage trajectory and cell state in the human lung |
| url | https://doi.org/10.1038/s12276-023-01076-z |
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