SPRC Suppresses Experimental Periodontitis by Modulating Th17/Treg Imbalance
Object: The aims of the study were to explore the protective effects of S-propargyl-cysteine (SPRC) on periodontitis and to determine the underlying mechanisms.Methods: A rat periodontitis model was constructed by injecting LPS and SPRC (0, 25, and 50 mg/kg/d) was administered intraperitoneally. H2S...
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Frontiers Media S.A.
2022-01-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2021.737334/full |
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| author | Qian Peng Qian Peng Bingkun Zhao Jie Lin Haixia Liu Rong Zhou Dongmei Lan Chao Yao Shaohua Cong Shen Tao Yizhun Zhu Raorao Wang Shengcai Qi Shengcai Qi |
| author_facet | Qian Peng Qian Peng Bingkun Zhao Jie Lin Haixia Liu Rong Zhou Dongmei Lan Chao Yao Shaohua Cong Shen Tao Yizhun Zhu Raorao Wang Shengcai Qi Shengcai Qi |
| author_sort | Qian Peng |
| collection | DOAJ |
| description | Object: The aims of the study were to explore the protective effects of S-propargyl-cysteine (SPRC) on periodontitis and to determine the underlying mechanisms.Methods: A rat periodontitis model was constructed by injecting LPS and SPRC (0, 25, and 50 mg/kg/d) was administered intraperitoneally. H2S and CSE level were detected. The alveolar bone level was evaluated by micro-CT, HE staining and methylene blue staining analysis. Inflammation-related factors, Treg and Th17 cells were detected by immunohistochemistry, RT-PCR, immunofluorescence, Western blot and flow cytometry. Phosphorylation levels of ERK1/2 and CREB were analysed.Results: The administration of SPRC significantly increased the expression of CSE in the gingival tissue and the concentration of endogenous H2S in the peripheral blood. Simultaneously, SPRC significantly inhibited the resorption of alveolar bone based on the H&E staining, micro-CT and methylene blue staining analysis. Compared with the periodontitis group, the levels of IL-17A, IL-10 were downregulated and IL-6,TGF-β1 were upregulated in the SPRC groups. In the SPRC group, the percentage of TH17 cells and the expression of ROR-γt were downregulated, while the percentage of Tregs and the expression of Foxp3 were upregulated accompanied with inhibition of phosphorylation ERK1/2 and CREB.Conclusion: SPRC can prevent the progression of periodontitis by regulating the Th17/Treg balance by inhibition of the ERK/CREB signalling pathway. |
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| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-18T04:18:17Z |
| publishDate | 2022-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-a54f8d0b1afd4cffa55c00f1e594898a2022-12-21T21:21:18ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852022-01-01910.3389/fbioe.2021.737334737334SPRC Suppresses Experimental Periodontitis by Modulating Th17/Treg ImbalanceQian Peng0Qian Peng1Bingkun Zhao2Jie Lin3Haixia Liu4Rong Zhou5Dongmei Lan6Chao Yao7Shaohua Cong8Shen Tao9Yizhun Zhu10Raorao Wang11Shengcai Qi12Shengcai Qi13Department of Stomatology, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai, ChinaHubei No. 3 People’s Hospital of Jianghan University, Wuhan, ChinaDepartment of Stomatology, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai, ChinaPharmacy Department, Minda Hospital of Hubei Minzu University, Enshi, ChinaDepartment of Stomatology, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai, ChinaDepartment of Stomatology, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai, ChinaMedical College of Anhui University of Science and Technology, Huainan, ChinaMedical College of Anhui University of Science and Technology, Huainan, ChinaJiading Central Hospital, Shanghai University of Medicine and Health Sciences, Shanghai, ChinaThe First People’s Hospital of KunShan, Kunshan, ChinaState Key Laboratory of Quality Research in Chinese Medicine and School of Pharmacy, Macau University of Science and Technology, Taipa, Macao SAR,ChinaDepartment of Stomatology, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai, ChinaDepartment of Stomatology, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai, ChinaDepartment of Prothodontics, Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases, Shanghai Stomatological Hospital, Fudan University, Shanghai, ChinaObject: The aims of the study were to explore the protective effects of S-propargyl-cysteine (SPRC) on periodontitis and to determine the underlying mechanisms.Methods: A rat periodontitis model was constructed by injecting LPS and SPRC (0, 25, and 50 mg/kg/d) was administered intraperitoneally. H2S and CSE level were detected. The alveolar bone level was evaluated by micro-CT, HE staining and methylene blue staining analysis. Inflammation-related factors, Treg and Th17 cells were detected by immunohistochemistry, RT-PCR, immunofluorescence, Western blot and flow cytometry. Phosphorylation levels of ERK1/2 and CREB were analysed.Results: The administration of SPRC significantly increased the expression of CSE in the gingival tissue and the concentration of endogenous H2S in the peripheral blood. Simultaneously, SPRC significantly inhibited the resorption of alveolar bone based on the H&E staining, micro-CT and methylene blue staining analysis. Compared with the periodontitis group, the levels of IL-17A, IL-10 were downregulated and IL-6,TGF-β1 were upregulated in the SPRC groups. In the SPRC group, the percentage of TH17 cells and the expression of ROR-γt were downregulated, while the percentage of Tregs and the expression of Foxp3 were upregulated accompanied with inhibition of phosphorylation ERK1/2 and CREB.Conclusion: SPRC can prevent the progression of periodontitis by regulating the Th17/Treg balance by inhibition of the ERK/CREB signalling pathway.https://www.frontiersin.org/articles/10.3389/fbioe.2021.737334/fullS-propargyl-cysteineperiodontitisTh17 cellsregulatory T cellsERK/CREB signalling pathway |
| spellingShingle | Qian Peng Qian Peng Bingkun Zhao Jie Lin Haixia Liu Rong Zhou Dongmei Lan Chao Yao Shaohua Cong Shen Tao Yizhun Zhu Raorao Wang Shengcai Qi Shengcai Qi SPRC Suppresses Experimental Periodontitis by Modulating Th17/Treg Imbalance Frontiers in Bioengineering and Biotechnology S-propargyl-cysteine periodontitis Th17 cells regulatory T cells ERK/CREB signalling pathway |
| title | SPRC Suppresses Experimental Periodontitis by Modulating Th17/Treg Imbalance |
| title_full | SPRC Suppresses Experimental Periodontitis by Modulating Th17/Treg Imbalance |
| title_fullStr | SPRC Suppresses Experimental Periodontitis by Modulating Th17/Treg Imbalance |
| title_full_unstemmed | SPRC Suppresses Experimental Periodontitis by Modulating Th17/Treg Imbalance |
| title_short | SPRC Suppresses Experimental Periodontitis by Modulating Th17/Treg Imbalance |
| title_sort | sprc suppresses experimental periodontitis by modulating th17 treg imbalance |
| topic | S-propargyl-cysteine periodontitis Th17 cells regulatory T cells ERK/CREB signalling pathway |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2021.737334/full |
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