Rosa laevigata Attenuates Allergic Asthma Exacerbated by Water-Soluble PM by Downregulating the MAPK Pathway
Exposure to water-soluble particulate matter (WPM) containing heavy metals can cause severe inflammatory responses and trigger and exacerbate the onset of asthma. As a follow-up study of Rosa laevigata (RL), this study analyzed the therapeutic effects and mechanisms of oral and intratracheal adminis...
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2022.925502/full |
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| author | Hyun Min Ko Hyun Min Ko Seung-Han Choi Seung-Han Choi Wona Jee Wona Jee Seung-Hyeon Lee Seung-Hyeon Lee Doil Park Doil Park Ji Hoon Jung Ji Hoon Jung Beom-Joon Lee Beom-Joon Lee Kwan-Il Kim Kwan-Il Kim Hee-Jae Jung Hee-Jae Jung Hyeung-Jin Jang Hyeung-Jin Jang |
| author_facet | Hyun Min Ko Hyun Min Ko Seung-Han Choi Seung-Han Choi Wona Jee Wona Jee Seung-Hyeon Lee Seung-Hyeon Lee Doil Park Doil Park Ji Hoon Jung Ji Hoon Jung Beom-Joon Lee Beom-Joon Lee Kwan-Il Kim Kwan-Il Kim Hee-Jae Jung Hee-Jae Jung Hyeung-Jin Jang Hyeung-Jin Jang |
| author_sort | Hyun Min Ko |
| collection | DOAJ |
| description | Exposure to water-soluble particulate matter (WPM) containing heavy metals can cause severe inflammatory responses and trigger and exacerbate the onset of asthma. As a follow-up study of Rosa laevigata (RL), this study analyzed the therapeutic effects and mechanisms of oral and intratracheal administration of RL and demonstrated anti-inflammatory effects in asthma models. Worse T-helper cell type 2 (Th2)-related inflammatory and pro-inflammatory responses were observed after simultaneous challenge with ovalbumin (OVA) and WPM. To establish a model of asthma exacerbated by WPM, BALB/c mice were sensitized with OVA + aluminum hydroxide and challenged with OVA + WPM. To confirm the therapeutic efficacy of RL, it was administered both orally and intratracheally. Histopathological analysis of H&E staining confirmed that oral and intratracheal administration of RL alleviated inflammatory cell infiltration in the airways aggravated by OVA + WPM. RL effectively reduced the number of inflammatory cells obtained from the bronchoalveolar lavage fluid. In addition, enzyme-linked immunosorbent assay (ELISA) and multiplex analysis of serum samples confirmed that the administration of RL reduced the levels of immuno-globulin E (IgE), Th2-related cytokines, and pro-inflammatory cytokines. Furthermore, real-time PCR analysis of lung tissue samples confirmed that the release of MUC5AC (Mucin 5AC, Oligomeric Mucus/Gel-Forming) and pro-inflammatory cytokines was reduced by RL, and western blotting confirmed that the administration of RL reduced the phosphorylation of ERK and p38 in the MAPK pathway. In conclusion, oral and intratracheal administration of RL appears to have an anti-asthmatic effect by reducing the secretion of Th2-related cytokines, pro-inflammatory cytokines, and IgE by downregulating the MAPK pathway. Thus, RL has further demonstrated potential for development as an oral and inhaled therapeutic for asthma symptoms exacerbated by WPM exposure. |
| first_indexed | 2024-12-12T06:29:10Z |
| format | Article |
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| language | English |
| last_indexed | 2024-12-12T06:29:10Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Pharmacology |
| spelling | doaj.art-a564c68652d04ec18d028e79556e6df52022-12-22T00:34:39ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122022-06-011310.3389/fphar.2022.925502925502Rosa laevigata Attenuates Allergic Asthma Exacerbated by Water-Soluble PM by Downregulating the MAPK PathwayHyun Min Ko0Hyun Min Ko1Seung-Han Choi2Seung-Han Choi3Wona Jee4Wona Jee5Seung-Hyeon Lee6Seung-Hyeon Lee7Doil Park8Doil Park9Ji Hoon Jung10Ji Hoon Jung11Beom-Joon Lee12Beom-Joon Lee13Kwan-Il Kim14Kwan-Il Kim15Hee-Jae Jung16Hee-Jae Jung17Hyeung-Jin Jang18Hyeung-Jin Jang19College of Korean Medicine, Kyung Hee University, Seoul, South KoreaDepartment of Science in Korean Medicine, Graduate School, Kyung Hee University, Seoul, South KoreaCollege of Korean Medicine, Kyung Hee University, Seoul, South KoreaDepartment of Biological Science in Korean Medicine, Graduate School, Kyung Hee University, Seoul, South KoreaCollege of Korean Medicine, Kyung Hee University, Seoul, South KoreaDepartment of Science in Korean Medicine, Graduate School, Kyung Hee University, Seoul, South KoreaCollege of Korean Medicine, Kyung Hee University, Seoul, South KoreaDepartment of Science in Korean Medicine, Graduate School, Kyung Hee University, Seoul, South KoreaCollege of Korean Medicine, Kyung Hee University, Seoul, South KoreaDepartment of Science in Korean Medicine, Graduate School, Kyung Hee University, Seoul, South KoreaCollege of Korean Medicine, Kyung Hee University, Seoul, South KoreaDepartment of Science in Korean Medicine, Graduate School, Kyung Hee University, Seoul, South KoreaDepartment of Biological Science in Korean Medicine, Graduate School, Kyung Hee University, Seoul, South KoreaDivision of Allergy, Immune and Respiratory System, Department of Internal Medicine, College of Korean Medicine, Kyung Hee University, Seoul, South KoreaDivision of Allergy, Immune and Respiratory System, Department of Internal Medicine, College of Korean Medicine, Kyung Hee University, Seoul, South KoreaDepartment of Clinical Korean Medicine, Graduate School, Kyung Hee University, Seoul, South KoreaDivision of Allergy, Immune and Respiratory System, Department of Internal Medicine, College of Korean Medicine, Kyung Hee University, Seoul, South KoreaDepartment of Clinical Korean Medicine, Graduate School, Kyung Hee University, Seoul, South KoreaCollege of Korean Medicine, Kyung Hee University, Seoul, South KoreaDepartment of Science in Korean Medicine, Graduate School, Kyung Hee University, Seoul, South KoreaExposure to water-soluble particulate matter (WPM) containing heavy metals can cause severe inflammatory responses and trigger and exacerbate the onset of asthma. As a follow-up study of Rosa laevigata (RL), this study analyzed the therapeutic effects and mechanisms of oral and intratracheal administration of RL and demonstrated anti-inflammatory effects in asthma models. Worse T-helper cell type 2 (Th2)-related inflammatory and pro-inflammatory responses were observed after simultaneous challenge with ovalbumin (OVA) and WPM. To establish a model of asthma exacerbated by WPM, BALB/c mice were sensitized with OVA + aluminum hydroxide and challenged with OVA + WPM. To confirm the therapeutic efficacy of RL, it was administered both orally and intratracheally. Histopathological analysis of H&E staining confirmed that oral and intratracheal administration of RL alleviated inflammatory cell infiltration in the airways aggravated by OVA + WPM. RL effectively reduced the number of inflammatory cells obtained from the bronchoalveolar lavage fluid. In addition, enzyme-linked immunosorbent assay (ELISA) and multiplex analysis of serum samples confirmed that the administration of RL reduced the levels of immuno-globulin E (IgE), Th2-related cytokines, and pro-inflammatory cytokines. Furthermore, real-time PCR analysis of lung tissue samples confirmed that the release of MUC5AC (Mucin 5AC, Oligomeric Mucus/Gel-Forming) and pro-inflammatory cytokines was reduced by RL, and western blotting confirmed that the administration of RL reduced the phosphorylation of ERK and p38 in the MAPK pathway. In conclusion, oral and intratracheal administration of RL appears to have an anti-asthmatic effect by reducing the secretion of Th2-related cytokines, pro-inflammatory cytokines, and IgE by downregulating the MAPK pathway. Thus, RL has further demonstrated potential for development as an oral and inhaled therapeutic for asthma symptoms exacerbated by WPM exposure.https://www.frontiersin.org/articles/10.3389/fphar.2022.925502/fullRosa laevigataparticulate matterasthmainflammationIgEcytokines |
| spellingShingle | Hyun Min Ko Hyun Min Ko Seung-Han Choi Seung-Han Choi Wona Jee Wona Jee Seung-Hyeon Lee Seung-Hyeon Lee Doil Park Doil Park Ji Hoon Jung Ji Hoon Jung Beom-Joon Lee Beom-Joon Lee Kwan-Il Kim Kwan-Il Kim Hee-Jae Jung Hee-Jae Jung Hyeung-Jin Jang Hyeung-Jin Jang Rosa laevigata Attenuates Allergic Asthma Exacerbated by Water-Soluble PM by Downregulating the MAPK Pathway Frontiers in Pharmacology Rosa laevigata particulate matter asthma inflammation IgE cytokines |
| title | Rosa laevigata Attenuates Allergic Asthma Exacerbated by Water-Soluble PM by Downregulating the MAPK Pathway |
| title_full | Rosa laevigata Attenuates Allergic Asthma Exacerbated by Water-Soluble PM by Downregulating the MAPK Pathway |
| title_fullStr | Rosa laevigata Attenuates Allergic Asthma Exacerbated by Water-Soluble PM by Downregulating the MAPK Pathway |
| title_full_unstemmed | Rosa laevigata Attenuates Allergic Asthma Exacerbated by Water-Soluble PM by Downregulating the MAPK Pathway |
| title_short | Rosa laevigata Attenuates Allergic Asthma Exacerbated by Water-Soluble PM by Downregulating the MAPK Pathway |
| title_sort | rosa laevigata attenuates allergic asthma exacerbated by water soluble pm by downregulating the mapk pathway |
| topic | Rosa laevigata particulate matter asthma inflammation IgE cytokines |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2022.925502/full |
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