Fine-grained, nonlinear registration of live cell movies reveals spatiotemporal organization of diffuse molecular processes.

We present an application of nonlinear image registration to align in microscopy time lapse sequences for every frame the cell outline and interior with the outline and interior of the same cell in a reference frame. The registration relies on a subcellular fiducial marker, a cell motion mask, and a...

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Bibliographic Details
Main Authors: Xuexia Jiang, Tadamoto Isogai, Joseph Chi, Gaudenz Danuser
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2022-12-01
Series:PLoS Computational Biology
Online Access:https://doi.org/10.1371/journal.pcbi.1009667