Effect of Sodium Nitrite, Nisin and Lactic Acid on the Prevalence and Antibiotic Resistance Patterns of <i>Listeria monocytogenes</i> Naturally Present in Poultry
The impact of treating minced chicken meat with sodium nitrite (SN, 100 ppm), nisin (Ni, 10 ppm) and lactic acid (LA, 3000 ppm) on the levels of some microbial groups indicating hygiene quality were investigated. Specifically, aerobic plate counts and culture-based counts of psychrotrophic microorga...
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| Format: | Article |
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MDPI AG
2023-08-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/17/3273 |
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| author | Cristina Rodríguez-Melcón Alexandra Esteves Javier Carballo Carlos Alonso-Calleja Rosa Capita |
| author_facet | Cristina Rodríguez-Melcón Alexandra Esteves Javier Carballo Carlos Alonso-Calleja Rosa Capita |
| author_sort | Cristina Rodríguez-Melcón |
| collection | DOAJ |
| description | The impact of treating minced chicken meat with sodium nitrite (SN, 100 ppm), nisin (Ni, 10 ppm) and lactic acid (LA, 3000 ppm) on the levels of some microbial groups indicating hygiene quality were investigated. Specifically, aerobic plate counts and culture-based counts of psychrotrophic microorganisms and enterobacteria were obtained. Additionally, the prevalence of <i>Listeria monocytogenes</i> and the resistance of 245 isolates from this bacterium to 15 antibiotics were documented. <i>L. monocytogenes</i> was isolated using the ISO 11290-1:2017 method and confirmed with polymerase chain reaction using the <i>lmo1030</i> gene. Antibiotic resistance was established using the disc diffusion technique (EUCAST and CLSI criteria). Twenty-four hours after treatment, the microbial load (log<sub>10</sub> cfu/g) was reduced (<i>p</i> < 0.05) relative to controls in those samples treated with LA, with counts of 5.51 ± 1.05 (LA-treated samples) vs. 7.53 ± 1.02 (control) for APC, 5.59 ± 1.14 (LA) vs. 7.13 ± 1.07 (control) for psychrotrophic microorganisms and 2.33 ± 0.51 (LA) vs. 4.23 ± 0.88 (control) for enterobacteria. <i>L. monocytogenes</i> was detected in 70% (control samples), 60% (samples receiving SN), 65% (Ni) and 50% (LA) (<i>p</i> > 0.05) of samples. All strains showed resistance to multiple antimicrobials (between 3 and 12). In all, 225 isolates (91.8%) showed a multi-drug resistant (MDR) phenotype, and one isolate (0.4%) showed an extensively drug-resistant (XDR) phenotype. The mean number of resistances per strain was lower (<i>p</i> < 0.01) in the control samples, at 5.77 ± 1.22, than in those receiving treatment, at 6.39 ± 1.51. It is suggested that the use of food additives might increase the prevalence of resistance to antibiotics in <i>L. monocytogenes</i>, although additional studies would be necessary to verify this finding by analyzing a higher number of samples and different foodstuffs and by increasing the number of antimicrobial compounds and concentrations to be tested. |
| first_indexed | 2024-03-10T23:23:55Z |
| format | Article |
| id | doaj.art-a581913d03534506a6eb34160305ee39 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T23:23:55Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-a581913d03534506a6eb34160305ee392023-11-19T08:09:16ZengMDPI AGFoods2304-81582023-08-011217327310.3390/foods12173273Effect of Sodium Nitrite, Nisin and Lactic Acid on the Prevalence and Antibiotic Resistance Patterns of <i>Listeria monocytogenes</i> Naturally Present in PoultryCristina Rodríguez-Melcón0Alexandra Esteves1Javier Carballo2Carlos Alonso-Calleja3Rosa Capita4Department of Food Hygiene and Technology, Veterinary Faculty, University of León, E-24071 León, SpainDepartment of Veterinary Sciences, School of Agrarian and Veterinary Sciences, University of Trás-os-Montes e Alto Douro, 5000-801 Vila Real, PortugalArea of Food Technology, Faculty of Sciences, University of Vigo, E-32004 Ourense, SpainDepartment of Food Hygiene and Technology, Veterinary Faculty, University of León, E-24071 León, SpainDepartment of Food Hygiene and Technology, Veterinary Faculty, University of León, E-24071 León, SpainThe impact of treating minced chicken meat with sodium nitrite (SN, 100 ppm), nisin (Ni, 10 ppm) and lactic acid (LA, 3000 ppm) on the levels of some microbial groups indicating hygiene quality were investigated. Specifically, aerobic plate counts and culture-based counts of psychrotrophic microorganisms and enterobacteria were obtained. Additionally, the prevalence of <i>Listeria monocytogenes</i> and the resistance of 245 isolates from this bacterium to 15 antibiotics were documented. <i>L. monocytogenes</i> was isolated using the ISO 11290-1:2017 method and confirmed with polymerase chain reaction using the <i>lmo1030</i> gene. Antibiotic resistance was established using the disc diffusion technique (EUCAST and CLSI criteria). Twenty-four hours after treatment, the microbial load (log<sub>10</sub> cfu/g) was reduced (<i>p</i> < 0.05) relative to controls in those samples treated with LA, with counts of 5.51 ± 1.05 (LA-treated samples) vs. 7.53 ± 1.02 (control) for APC, 5.59 ± 1.14 (LA) vs. 7.13 ± 1.07 (control) for psychrotrophic microorganisms and 2.33 ± 0.51 (LA) vs. 4.23 ± 0.88 (control) for enterobacteria. <i>L. monocytogenes</i> was detected in 70% (control samples), 60% (samples receiving SN), 65% (Ni) and 50% (LA) (<i>p</i> > 0.05) of samples. All strains showed resistance to multiple antimicrobials (between 3 and 12). In all, 225 isolates (91.8%) showed a multi-drug resistant (MDR) phenotype, and one isolate (0.4%) showed an extensively drug-resistant (XDR) phenotype. The mean number of resistances per strain was lower (<i>p</i> < 0.01) in the control samples, at 5.77 ± 1.22, than in those receiving treatment, at 6.39 ± 1.51. It is suggested that the use of food additives might increase the prevalence of resistance to antibiotics in <i>L. monocytogenes</i>, although additional studies would be necessary to verify this finding by analyzing a higher number of samples and different foodstuffs and by increasing the number of antimicrobial compounds and concentrations to be tested.https://www.mdpi.com/2304-8158/12/17/3273chicken meathygienic quality<i>Listeria monocytogenes</i>antibiotic resistancefood additives |
| spellingShingle | Cristina Rodríguez-Melcón Alexandra Esteves Javier Carballo Carlos Alonso-Calleja Rosa Capita Effect of Sodium Nitrite, Nisin and Lactic Acid on the Prevalence and Antibiotic Resistance Patterns of <i>Listeria monocytogenes</i> Naturally Present in Poultry Foods chicken meat hygienic quality <i>Listeria monocytogenes</i> antibiotic resistance food additives |
| title | Effect of Sodium Nitrite, Nisin and Lactic Acid on the Prevalence and Antibiotic Resistance Patterns of <i>Listeria monocytogenes</i> Naturally Present in Poultry |
| title_full | Effect of Sodium Nitrite, Nisin and Lactic Acid on the Prevalence and Antibiotic Resistance Patterns of <i>Listeria monocytogenes</i> Naturally Present in Poultry |
| title_fullStr | Effect of Sodium Nitrite, Nisin and Lactic Acid on the Prevalence and Antibiotic Resistance Patterns of <i>Listeria monocytogenes</i> Naturally Present in Poultry |
| title_full_unstemmed | Effect of Sodium Nitrite, Nisin and Lactic Acid on the Prevalence and Antibiotic Resistance Patterns of <i>Listeria monocytogenes</i> Naturally Present in Poultry |
| title_short | Effect of Sodium Nitrite, Nisin and Lactic Acid on the Prevalence and Antibiotic Resistance Patterns of <i>Listeria monocytogenes</i> Naturally Present in Poultry |
| title_sort | effect of sodium nitrite nisin and lactic acid on the prevalence and antibiotic resistance patterns of i listeria monocytogenes i naturally present in poultry |
| topic | chicken meat hygienic quality <i>Listeria monocytogenes</i> antibiotic resistance food additives |
| url | https://www.mdpi.com/2304-8158/12/17/3273 |
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