Analysis of contaminated microorganisms in commercial honey
ObjectiveTo investigate the microbial contamination in 54 batches of commercial honey.MethodsAerobic plate colony counts for bacteria and colonies of mould and osmophilic yeasts in honey were determined according to the National Food Safety Standard. The bacteria and fungi in unqualified samples wer...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | zho |
| Published: |
Shanghai Preventive Medicine Association
2022-01-01
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| Series: | Shanghai yufang yixue |
| Subjects: | |
| Online Access: | http://www.sjpm.org.cn/article/doi/10.19428/j.cnki.sjpm.2022.21087 |
| _version_ | 1797939250808750080 |
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| author | JIANG Bo LI Qiongqiong ZHANG Zhihua DAI Jiali QIN Feng YANG Meicheng |
| author_facet | JIANG Bo LI Qiongqiong ZHANG Zhihua DAI Jiali QIN Feng YANG Meicheng |
| author_sort | JIANG Bo |
| collection | DOAJ |
| description | ObjectiveTo investigate the microbial contamination in 54 batches of commercial honey.MethodsAerobic plate colony counts for bacteria and colonies of mould and osmophilic yeasts in honey were determined according to the National Food Safety Standard. The bacteria and fungi in unqualified samples were further identified and analyzed by morphology， MALDI-TOF-MS and large subunit （LSU） rRNA gene sequence.ResultsThree unqualified batches were found. One batch had aerobic plate colony counts exceeding the standard， with a variety of bacteria including Bacillus sp.， Paenibacillus sp. and Brevibacillus sp. Two batches had mould counts exceeding the standard. Aspergillus sp was detected in both samples （from the same manufacturer）， and the DNA sequence homology was very high， suggesting the mould might come from the same pollution source.ConclusionThere are many kinds of microbial contamination in honey. Manufacturers should strengthen the microbe control in monitoring process to avoid the repeated microbial contamination. |
| first_indexed | 2024-04-10T19:13:43Z |
| format | Article |
| id | doaj.art-a58899d38de44ce5a78838dcaec040f8 |
| institution | Directory Open Access Journal |
| issn | 1004-9231 |
| language | zho |
| last_indexed | 2024-04-10T19:13:43Z |
| publishDate | 2022-01-01 |
| publisher | Shanghai Preventive Medicine Association |
| record_format | Article |
| series | Shanghai yufang yixue |
| spelling | doaj.art-a58899d38de44ce5a78838dcaec040f82023-01-30T10:51:57ZzhoShanghai Preventive Medicine AssociationShanghai yufang yixue1004-92312022-01-01341778010.19428/j.cnki.sjpm.2022.210871004-9231（2022）01-0077-04Analysis of contaminated microorganisms in commercial honeyJIANG BoLI QiongqiongZHANG ZhihuaDAI JialiQIN FengYANG MeichengObjectiveTo investigate the microbial contamination in 54 batches of commercial honey.MethodsAerobic plate colony counts for bacteria and colonies of mould and osmophilic yeasts in honey were determined according to the National Food Safety Standard. The bacteria and fungi in unqualified samples were further identified and analyzed by morphology， MALDI-TOF-MS and large subunit （LSU） rRNA gene sequence.ResultsThree unqualified batches were found. One batch had aerobic plate colony counts exceeding the standard， with a variety of bacteria including Bacillus sp.， Paenibacillus sp. and Brevibacillus sp. Two batches had mould counts exceeding the standard. Aspergillus sp was detected in both samples （from the same manufacturer）， and the DNA sequence homology was very high， suggesting the mould might come from the same pollution source.ConclusionThere are many kinds of microbial contamination in honey. Manufacturers should strengthen the microbe control in monitoring process to avoid the repeated microbial contamination.http://www.sjpm.org.cn/article/doi/10.19428/j.cnki.sjpm.2022.21087honeymicrobial contaminationprocess controlidentification and analysis |
| spellingShingle | JIANG Bo LI Qiongqiong ZHANG Zhihua DAI Jiali QIN Feng YANG Meicheng Analysis of contaminated microorganisms in commercial honey Shanghai yufang yixue honey microbial contamination process control identification and analysis |
| title | Analysis of contaminated microorganisms in commercial honey |
| title_full | Analysis of contaminated microorganisms in commercial honey |
| title_fullStr | Analysis of contaminated microorganisms in commercial honey |
| title_full_unstemmed | Analysis of contaminated microorganisms in commercial honey |
| title_short | Analysis of contaminated microorganisms in commercial honey |
| title_sort | analysis of contaminated microorganisms in commercial honey |
| topic | honey microbial contamination process control identification and analysis |
| url | http://www.sjpm.org.cn/article/doi/10.19428/j.cnki.sjpm.2022.21087 |
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