Recombination-mediated genetic engineering of a bacterial artificial chromosome clone of modified vaccinia virus Ankara (MVA).

The production, manipulation and rescue of a bacterial artificial chromosome clone of Vaccinia virus (VAC-BAC) in order to expedite construction of expression vectors and mutagenesis of the genome has been described (Domi & Moss, 2002, PNAS99 12415-20). The genomic BAC clone was 'rescued�...

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Bibliographic Details
Main Authors: Matthew G Cottingham, Rikke F Andersen, Alexandra J Spencer, Saroj Saurya, Julie Furze, Adrian V S Hill, Sarah C Gilbert
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2008-02-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC2242847?pdf=render