Direct Detection of Glutathione Biosynthesis, Conjugation, Depletion and Recovery in Intact Hepatoma Cells

Nuclear magnetic resonance (NMR) spectroscopy was used to monitor glutathione metabolism in alginate-encapsulated JM-1 hepatoma cells perfused with growth media containing [3,3′-<sup>13</sup>C<sub>2</sub>]-cystine. After 20 h of perfusion with labeled medium, the <sup>1...

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Bibliographic Details
Main Authors: Rex E. Jeffries, Shawn M. Gomez, Jeffrey M. Macdonald, Michael P. Gamcsik
Format: Article
Language:English
Published: MDPI AG 2022-04-01
Series:International Journal of Molecular Sciences
Subjects:
Online Access:https://www.mdpi.com/1422-0067/23/9/4733