A photocontrolled one-pot isothermal amplification and CRISPR-Cas12a assay for rapid detection of SARS-CoV-2 Omicron variants

A photocontrolled one-pot isothermal amplification and CRISPR-Cas12a assay for rapid detection of SARS-CoV-2 Omicron variants

ABSTRACTCRISPR-Cas technology has widely been applied to detect single-nucleotide mutation and is considered as the next generation of molecular diagnostics. We previously reported the combination of nucleic acid amplification (NAA) and CRISPR-Cas12a system to distinguish major severe acute respirat...

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Bibliographic Details
Main Authors: Qian Sun, Hongqing Lin, Yuan Li, Liping Yuan, Baisheng Li, Yunan Ma, Haiying Wang, Xiaoling Deng, Hongliang Chen, Shixing Tang
Format: Article
Language:English
Published: American Society for Microbiology 2024-03-01
Series:Microbiology Spectrum
Subjects:
CRISPR-Cas12a
photocleavage
isothermal amplification
one-pot assay
SARS-CoV-2
Omicron variant
Online Access:https://journals.asm.org/doi/10.1128/spectrum.03645-23

Internet

https://journals.asm.org/doi/10.1128/spectrum.03645-23

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