Chronic Mild Stress and Venlafaxine Treatment Were Associated with Altered Expression Level and Methylation Status of New Candidate Inflammatory Genes in PBMCs and Brain Structures of Wistar Rats
Preclinical studies conducted to date suggest that depression could be elicited by the elevated expression of proinflammatory molecules: these play a key role in the mediation of neurochemical, neuroendocrine and behavioral changes. Thus, this study investigates the effect of chronic mild stress (CM...
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MDPI AG
2021-04-01
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| author | Katarzyna Bialek Piotr Czarny Paulina Wigner Ewelina Synowiec Gabriela Barszczewska Michal Bijak Janusz Szemraj Monika Niemczyk Katarzyna Tota-Glowczyk Mariusz Papp Tomasz Sliwinski |
| author_facet | Katarzyna Bialek Piotr Czarny Paulina Wigner Ewelina Synowiec Gabriela Barszczewska Michal Bijak Janusz Szemraj Monika Niemczyk Katarzyna Tota-Glowczyk Mariusz Papp Tomasz Sliwinski |
| author_sort | Katarzyna Bialek |
| collection | DOAJ |
| description | Preclinical studies conducted to date suggest that depression could be elicited by the elevated expression of proinflammatory molecules: these play a key role in the mediation of neurochemical, neuroendocrine and behavioral changes. Thus, this study investigates the effect of chronic mild stress (CMS) and administration of venlafaxine (SSRI) on the expression and methylation status of new target inflammatory genes: TGFA, TGFB, IRF1, PTGS2 and IKBKB, in peripheral blood mononuclear cells (PMBCs) and in selected brain structures of rats. Adult male Wistar rats were subjected to the CMS and further divided into matched subgroups to receive vehicle or venlafaxine. TaqMan gene expression assay and methylation-sensitive high-resolution melting (MS-HRM) were used to evaluate the expression of the genes and the methylation status of their promoters, respectively. Our results indicate that both CMS and chronic treatment with venlafaxine were associated with changes in expression of the studied genes and their promoter methylation status in PMBCs and the brain. Moreover, the effect of antidepressant administration clearly differed between brain structures. Summarizing, our results confirm at least a partial association between TGFA, TGFB, IRF1, PTGS2 and IKBKB and depressive disorders. |
| first_indexed | 2024-03-10T11:50:23Z |
| format | Article |
| id | doaj.art-a5a6be88053e4635982060bf5d07287f |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T11:50:23Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-a5a6be88053e4635982060bf5d07287f2023-11-21T17:45:04ZengMDPI AGGenes2073-44252021-04-0112566710.3390/genes12050667Chronic Mild Stress and Venlafaxine Treatment Were Associated with Altered Expression Level and Methylation Status of New Candidate Inflammatory Genes in PBMCs and Brain Structures of Wistar RatsKatarzyna Bialek0Piotr Czarny1Paulina Wigner2Ewelina Synowiec3Gabriela Barszczewska4Michal Bijak5Janusz Szemraj6Monika Niemczyk7Katarzyna Tota-Glowczyk8Mariusz Papp9Tomasz Sliwinski10Laboratory of Medical Genetics, Faculty of Biology and Environmental Protection, University of Lodz, 90-236 Lodz, PolandDepartment of Medical Biochemistry, Medical University of Lodz, 92-216 Lodz, PolandDepartment of General Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, 90-236 Lodz, PolandLaboratory of Medical Genetics, Faculty of Biology and Environmental Protection, University of Lodz, 90-236 Lodz, PolandLaboratory of Medical Genetics, Faculty of Biology and Environmental Protection, University of Lodz, 90-236 Lodz, PolandBiohazard Prevention Centre, Faculty of Biology and Environmental Protection, University of Lodz, 90-236 Lodz, PolandDepartment of Medical Biochemistry, Medical University of Lodz, 92-216 Lodz, PolandInstitute of Pharmacology, Polish Academy of Sciences, 31-343 Krakow, PolandInstitute of Pharmacology, Polish Academy of Sciences, 31-343 Krakow, PolandInstitute of Pharmacology, Polish Academy of Sciences, 31-343 Krakow, PolandLaboratory of Medical Genetics, Faculty of Biology and Environmental Protection, University of Lodz, 90-236 Lodz, PolandPreclinical studies conducted to date suggest that depression could be elicited by the elevated expression of proinflammatory molecules: these play a key role in the mediation of neurochemical, neuroendocrine and behavioral changes. Thus, this study investigates the effect of chronic mild stress (CMS) and administration of venlafaxine (SSRI) on the expression and methylation status of new target inflammatory genes: TGFA, TGFB, IRF1, PTGS2 and IKBKB, in peripheral blood mononuclear cells (PMBCs) and in selected brain structures of rats. Adult male Wistar rats were subjected to the CMS and further divided into matched subgroups to receive vehicle or venlafaxine. TaqMan gene expression assay and methylation-sensitive high-resolution melting (MS-HRM) were used to evaluate the expression of the genes and the methylation status of their promoters, respectively. Our results indicate that both CMS and chronic treatment with venlafaxine were associated with changes in expression of the studied genes and their promoter methylation status in PMBCs and the brain. Moreover, the effect of antidepressant administration clearly differed between brain structures. Summarizing, our results confirm at least a partial association between TGFA, TGFB, IRF1, PTGS2 and IKBKB and depressive disorders.https://www.mdpi.com/2073-4425/12/5/667depressionchronic mild stressvenlafaxineinflammationexpressionmethylation |
| spellingShingle | Katarzyna Bialek Piotr Czarny Paulina Wigner Ewelina Synowiec Gabriela Barszczewska Michal Bijak Janusz Szemraj Monika Niemczyk Katarzyna Tota-Glowczyk Mariusz Papp Tomasz Sliwinski Chronic Mild Stress and Venlafaxine Treatment Were Associated with Altered Expression Level and Methylation Status of New Candidate Inflammatory Genes in PBMCs and Brain Structures of Wistar Rats Genes depression chronic mild stress venlafaxine inflammation expression methylation |
| title | Chronic Mild Stress and Venlafaxine Treatment Were Associated with Altered Expression Level and Methylation Status of New Candidate Inflammatory Genes in PBMCs and Brain Structures of Wistar Rats |
| title_full | Chronic Mild Stress and Venlafaxine Treatment Were Associated with Altered Expression Level and Methylation Status of New Candidate Inflammatory Genes in PBMCs and Brain Structures of Wistar Rats |
| title_fullStr | Chronic Mild Stress and Venlafaxine Treatment Were Associated with Altered Expression Level and Methylation Status of New Candidate Inflammatory Genes in PBMCs and Brain Structures of Wistar Rats |
| title_full_unstemmed | Chronic Mild Stress and Venlafaxine Treatment Were Associated with Altered Expression Level and Methylation Status of New Candidate Inflammatory Genes in PBMCs and Brain Structures of Wistar Rats |
| title_short | Chronic Mild Stress and Venlafaxine Treatment Were Associated with Altered Expression Level and Methylation Status of New Candidate Inflammatory Genes in PBMCs and Brain Structures of Wistar Rats |
| title_sort | chronic mild stress and venlafaxine treatment were associated with altered expression level and methylation status of new candidate inflammatory genes in pbmcs and brain structures of wistar rats |
| topic | depression chronic mild stress venlafaxine inflammation expression methylation |
| url | https://www.mdpi.com/2073-4425/12/5/667 |
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