Rapid Detection of Three Foodborne Pathogenic Bacteria in School Catering by Isothermal Multiple Self-matching-initiated Amplification Technique
Objective: In this paper, a detection method suitable for rapid identification of three pathogenic bacteria from food in the school catering was studied. Method: Based on isothermal multiple self-matching-initiated amplification technology (IMSA), strain-specific primers were designed for Salmonella...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2023-09-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022110196 |
| _version_ | 1797690085146099712 |
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| author | Qiang JIAO Wansheng CHEN Nan ZHOU Qingfa ZHANG Zhiyong LIU |
| author_facet | Qiang JIAO Wansheng CHEN Nan ZHOU Qingfa ZHANG Zhiyong LIU |
| author_sort | Qiang JIAO |
| collection | DOAJ |
| description | Objective: In this paper, a detection method suitable for rapid identification of three pathogenic bacteria from food in the school catering was studied. Method: Based on isothermal multiple self-matching-initiated amplification technology (IMSA), strain-specific primers were designed for Salmonella invA gene, Escherichia coli O157:H7/NM rfbE gene and Listeria monocytogenes prfA gene to test the sensitivity, specificity, shortest enrichment time, and minimum detection limit of bacterial content. Food microbiology inspection according to National Food Safety Standards (GB/T 4789.4-2016, GB/T 4789.30-2016, GB/T 4789.36-2016) were the reference methods，and compared the consistency of the two methods. Result: The results showed that the sensitivity of our method to Salmonella, Escherichia coli O157:H7/NM and Listeria monocytogenes were 2.14×103, 2.79×103 and 3.62×103 CFU/mL respectively, the specificity was 100%, the shortest enrichment time of artificial contamination were 6, 8 and 8 hours respectively. The detection limits of the lowest bacteria content were 2.14, 2.79 and 3.62 CFU/25 g respectively, and the consistency of the results of 74 food samples using IMSA method and the reference method was 100%. Conclusion: IMSA technology of pathogenic bacteria detection in food had the advantage of having high sensitivity, strong specificity and accurate results, which could complete the detection of three pathogenic bacteria in food in a short time and was suitable for the rapid identification of pathogenic bacteria in school catering. |
| first_indexed | 2024-03-12T01:54:27Z |
| format | Article |
| id | doaj.art-a5a9933141034ad38b201e3d5718a385 |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-03-12T01:54:27Z |
| publishDate | 2023-09-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-a5a9933141034ad38b201e3d5718a3852023-09-08T06:41:53ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062023-09-01441837137710.13386/j.issn1002-0306.20221101962022110196-18Rapid Detection of Three Foodborne Pathogenic Bacteria in School Catering by Isothermal Multiple Self-matching-initiated Amplification TechniqueQiang JIAO0Wansheng CHEN1Nan ZHOU2Qingfa ZHANG3Zhiyong LIU4Henan Province Food Inspection Research Institute, Key Laboratory of Food Safety Quick Testing and Smart Supervision Technology for State Regulation, Zhengzhou 450000, ChinaHenan Province Food Inspection Research Institute, Key Laboratory of Food Safety Quick Testing and Smart Supervision Technology for State Regulation, Zhengzhou 450000, ChinaHenan Province Food Inspection Research Institute, Key Laboratory of Food Safety Quick Testing and Smart Supervision Technology for State Regulation, Zhengzhou 450000, ChinaGuangzhou DI’AO Bio-technology Co., Ltd., Guangzhou 510507, ChinaHenan Province Food Inspection Research Institute, Key Laboratory of Food Safety Quick Testing and Smart Supervision Technology for State Regulation, Zhengzhou 450000, ChinaObjective: In this paper, a detection method suitable for rapid identification of three pathogenic bacteria from food in the school catering was studied. Method: Based on isothermal multiple self-matching-initiated amplification technology (IMSA), strain-specific primers were designed for Salmonella invA gene, Escherichia coli O157:H7/NM rfbE gene and Listeria monocytogenes prfA gene to test the sensitivity, specificity, shortest enrichment time, and minimum detection limit of bacterial content. Food microbiology inspection according to National Food Safety Standards (GB/T 4789.4-2016, GB/T 4789.30-2016, GB/T 4789.36-2016) were the reference methods，and compared the consistency of the two methods. Result: The results showed that the sensitivity of our method to Salmonella, Escherichia coli O157:H7/NM and Listeria monocytogenes were 2.14×103, 2.79×103 and 3.62×103 CFU/mL respectively, the specificity was 100%, the shortest enrichment time of artificial contamination were 6, 8 and 8 hours respectively. The detection limits of the lowest bacteria content were 2.14, 2.79 and 3.62 CFU/25 g respectively, and the consistency of the results of 74 food samples using IMSA method and the reference method was 100%. Conclusion: IMSA technology of pathogenic bacteria detection in food had the advantage of having high sensitivity, strong specificity and accurate results, which could complete the detection of three pathogenic bacteria in food in a short time and was suitable for the rapid identification of pathogenic bacteria in school catering.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022110196salmonellaescherichia coli o157:h7/nmlisteria monocytogenesisothermal multiple self-matching-initiated amplification |
| spellingShingle | Qiang JIAO Wansheng CHEN Nan ZHOU Qingfa ZHANG Zhiyong LIU Rapid Detection of Three Foodborne Pathogenic Bacteria in School Catering by Isothermal Multiple Self-matching-initiated Amplification Technique Shipin gongye ke-ji salmonella escherichia coli o157:h7/nm listeria monocytogenes isothermal multiple self-matching-initiated amplification |
| title | Rapid Detection of Three Foodborne Pathogenic Bacteria in School Catering by Isothermal Multiple Self-matching-initiated Amplification Technique |
| title_full | Rapid Detection of Three Foodborne Pathogenic Bacteria in School Catering by Isothermal Multiple Self-matching-initiated Amplification Technique |
| title_fullStr | Rapid Detection of Three Foodborne Pathogenic Bacteria in School Catering by Isothermal Multiple Self-matching-initiated Amplification Technique |
| title_full_unstemmed | Rapid Detection of Three Foodborne Pathogenic Bacteria in School Catering by Isothermal Multiple Self-matching-initiated Amplification Technique |
| title_short | Rapid Detection of Three Foodborne Pathogenic Bacteria in School Catering by Isothermal Multiple Self-matching-initiated Amplification Technique |
| title_sort | rapid detection of three foodborne pathogenic bacteria in school catering by isothermal multiple self matching initiated amplification technique |
| topic | salmonella escherichia coli o157:h7/nm listeria monocytogenes isothermal multiple self-matching-initiated amplification |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022110196 |
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