Reconstitution of Mycobacterium marinum Nonhomologous DNA End Joining Pathway in Leishmania

Reconstitution of Mycobacterium marinum Nonhomologous DNA End Joining Pathway in Leishmania

ABSTRACT In mammalian cells, DNA double-strand breaks (DSBs) are mainly repaired by nonhomologous end joining (NHEJ) pathway. Ku (a heterodimer formed by Ku70 and Ku80 proteins) and DNA ligase IV are the core NHEJ factors. Ku could also be involved in other cellular processes, including telomere len...

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Bibliographic Details
Main Authors: Wen-Wei Zhang, Douglas G. Wright, Lynn Harrison, Greg Matlashewski
Format: Article
Language:English
Published: American Society for Microbiology 2022-06-01
Series:mSphere
Subjects:
Leishmania donovani
CRISPR-Cas9
gene targeting
gene deletion
double-strand DNA break
DSB repair
Online Access:https://journals.asm.org/doi/10.1128/msphere.00156-22

Internet

https://journals.asm.org/doi/10.1128/msphere.00156-22

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