D-Allulose (D-Psicose) Biotransformation From Allitol by a Newly Found NAD(P)-Dependent Alcohol Dehydrogenase From Gluconobacter frateurii NBRC 3264 and the Enzyme Characterization

D-Allulose (D-Psicose) Biotransformation From Allitol by a Newly Found NAD(P)-Dependent Alcohol Dehydrogenase From Gluconobacter frateurii NBRC 3264 and the Enzyme Characterization

The NAD(P)-dependent alcohol dehydrogenase (ADH) gene was cloned from Gluconobacter frateurii NBRC 3264 and expressed in Escherichia coli BL21 star (DE3). The expressed enzyme was purified and the characteristics were investigated. The results showed that this ADH can convert allitol into D-allulose...

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Bibliographic Details
Main Authors: Xin Wen, Huibin Lin, Yuhang Ning, Guangwen Liu, Yilin Ren, Can Li, Chengjia Zhang, Jianqun Lin, Xin Song, Jianqiang Lin
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-04-01
Series:Frontiers in Microbiology
Subjects:
D-allulose
allitol
NAD(P)-dependent alcohol dehydrogenase
Gluconobacter frateurii NBRC 3264
biotransformation
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2022.870168/full

Internet

https://www.frontiersin.org/articles/10.3389/fmicb.2022.870168/full

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