Optimal reference genes for RNA tissue analysis in small animal models of hemorrhagic fever viruses

Abstract Reverse-transcription quantitative polymerase chain reaction assays are frequently used to evaluate gene expression in animal model studies. Data analyses depend on normalization using a suitable reference gene (RG) to minimize effects of variation due to sample collection, sample processin...

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Bibliographic Details
Main Authors: Katherine A. Davies, Stephen R. Welch, Teresa E. Sorvillo, JoAnn D. Coleman-McCray, María Laura Martin, Julia M. Brignone, Joel M. Montgomery, Christina F. Spiropoulou, Jessica R. Spengler
Format: Article
Language:English
Published: Nature Portfolio 2023-11-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-023-45740-w