Dual Network Hydrogels Incorporated with Bone Morphogenic Protein-7-Loaded Hyaluronic Acid Complex Nanoparticles for Inducing Chondrogenic Differentiation of Synovium-Derived Mesenchymal Stem Cells
Alginate-poloxamer (ALG-POL) copolymer with optimal POL content was synthesized, and it was combined with silk fibroin (SF) for building ALG-POL/SF dual network hydrogels. Hyaluronic acid(HA)/chitosan-poly(dioxanone)(CH-PDO) complex nanoparticles (NPs) with optimized composition and high encapsulati...
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| Format: | Article |
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MDPI AG
2020-06-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/12/7/613 |
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| author | Qing Min Jiaoyan Liu Yuchen Zhang Bin Yang Ying Wan Jiliang Wu |
| author_facet | Qing Min Jiaoyan Liu Yuchen Zhang Bin Yang Ying Wan Jiliang Wu |
| author_sort | Qing Min |
| collection | DOAJ |
| description | Alginate-poloxamer (ALG-POL) copolymer with optimal POL content was synthesized, and it was combined with silk fibroin (SF) for building ALG-POL/SF dual network hydrogels. Hyaluronic acid(HA)/chitosan-poly(dioxanone)(CH-PDO) complex nanoparticles (NPs) with optimized composition and high encapsulation efficiency were employed as a vehicle for loading bone morphogenic protein-7 (BMP-7). BMP-7-loaded HA/CH-PDO NPs were incorporated into ALG-POL/SF hydrogel for constructing composite gels to achieve controlled release of BMP-7. These gels showed thermosensitive sol-gel transitions near physiological temperature and pH; and they were tested to be elastic, tough and strong. Some gels exhibited abilities to administer the BMP-7 release in nearly linear manners for a few weeks. Synovium-derived mesenchymal stem cells (SMSCs) were seeded into optimally fabricated gels for assessing their chondrogenic differentiation potency. Real-time PCR analyses showed that the blank ALG-POL/SF gels were not able to induce the chondrogenic differentiation of SMSCs, whereas SMSCs were detected to significantly express cartilage-related genes once they were seeded in the BMP-7-loaded ALG-POL/SF gel for two weeks. The synthesis of cartilaginous matrix components further confirmed that SMSCs seeded in the BMP-7-loaded ALG-POL/SF gel differentiated toward chondrogenesis. Results suggest that BMP-7-loaded ALG-POL/SF composite gels can function as a promising biomaterial for cartilage tissue engineering applications. |
| first_indexed | 2024-03-10T18:46:41Z |
| format | Article |
| id | doaj.art-a5efd3d7fac244b1a020b07d7f330ab8 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-10T18:46:41Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-a5efd3d7fac244b1a020b07d7f330ab82023-11-20T05:28:03ZengMDPI AGPharmaceutics1999-49232020-06-0112761310.3390/pharmaceutics12070613Dual Network Hydrogels Incorporated with Bone Morphogenic Protein-7-Loaded Hyaluronic Acid Complex Nanoparticles for Inducing Chondrogenic Differentiation of Synovium-Derived Mesenchymal Stem CellsQing Min0Jiaoyan Liu1Yuchen Zhang2Bin Yang3Ying Wan4Jiliang Wu5School of Pharmacy, Hubei University of Science and Technology, Xianning 437100, ChinaCollege of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, ChinaSchool of Pharmacy, Hubei University of Science and Technology, Xianning 437100, ChinaCollege of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, ChinaCollege of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, ChinaSchool of Pharmacy, Hubei University of Science and Technology, Xianning 437100, ChinaAlginate-poloxamer (ALG-POL) copolymer with optimal POL content was synthesized, and it was combined with silk fibroin (SF) for building ALG-POL/SF dual network hydrogels. Hyaluronic acid(HA)/chitosan-poly(dioxanone)(CH-PDO) complex nanoparticles (NPs) with optimized composition and high encapsulation efficiency were employed as a vehicle for loading bone morphogenic protein-7 (BMP-7). BMP-7-loaded HA/CH-PDO NPs were incorporated into ALG-POL/SF hydrogel for constructing composite gels to achieve controlled release of BMP-7. These gels showed thermosensitive sol-gel transitions near physiological temperature and pH; and they were tested to be elastic, tough and strong. Some gels exhibited abilities to administer the BMP-7 release in nearly linear manners for a few weeks. Synovium-derived mesenchymal stem cells (SMSCs) were seeded into optimally fabricated gels for assessing their chondrogenic differentiation potency. Real-time PCR analyses showed that the blank ALG-POL/SF gels were not able to induce the chondrogenic differentiation of SMSCs, whereas SMSCs were detected to significantly express cartilage-related genes once they were seeded in the BMP-7-loaded ALG-POL/SF gel for two weeks. The synthesis of cartilaginous matrix components further confirmed that SMSCs seeded in the BMP-7-loaded ALG-POL/SF gel differentiated toward chondrogenesis. Results suggest that BMP-7-loaded ALG-POL/SF composite gels can function as a promising biomaterial for cartilage tissue engineering applications.https://www.mdpi.com/1999-4923/12/7/613dual network hydrogelcomplex nanoparticlesbone morphogenic protein-7chondrogenic differentiationsynovium-derived mesenchymal stem cells |
| spellingShingle | Qing Min Jiaoyan Liu Yuchen Zhang Bin Yang Ying Wan Jiliang Wu Dual Network Hydrogels Incorporated with Bone Morphogenic Protein-7-Loaded Hyaluronic Acid Complex Nanoparticles for Inducing Chondrogenic Differentiation of Synovium-Derived Mesenchymal Stem Cells Pharmaceutics dual network hydrogel complex nanoparticles bone morphogenic protein-7 chondrogenic differentiation synovium-derived mesenchymal stem cells |
| title | Dual Network Hydrogels Incorporated with Bone Morphogenic Protein-7-Loaded Hyaluronic Acid Complex Nanoparticles for Inducing Chondrogenic Differentiation of Synovium-Derived Mesenchymal Stem Cells |
| title_full | Dual Network Hydrogels Incorporated with Bone Morphogenic Protein-7-Loaded Hyaluronic Acid Complex Nanoparticles for Inducing Chondrogenic Differentiation of Synovium-Derived Mesenchymal Stem Cells |
| title_fullStr | Dual Network Hydrogels Incorporated with Bone Morphogenic Protein-7-Loaded Hyaluronic Acid Complex Nanoparticles for Inducing Chondrogenic Differentiation of Synovium-Derived Mesenchymal Stem Cells |
| title_full_unstemmed | Dual Network Hydrogels Incorporated with Bone Morphogenic Protein-7-Loaded Hyaluronic Acid Complex Nanoparticles for Inducing Chondrogenic Differentiation of Synovium-Derived Mesenchymal Stem Cells |
| title_short | Dual Network Hydrogels Incorporated with Bone Morphogenic Protein-7-Loaded Hyaluronic Acid Complex Nanoparticles for Inducing Chondrogenic Differentiation of Synovium-Derived Mesenchymal Stem Cells |
| title_sort | dual network hydrogels incorporated with bone morphogenic protein 7 loaded hyaluronic acid complex nanoparticles for inducing chondrogenic differentiation of synovium derived mesenchymal stem cells |
| topic | dual network hydrogel complex nanoparticles bone morphogenic protein-7 chondrogenic differentiation synovium-derived mesenchymal stem cells |
| url | https://www.mdpi.com/1999-4923/12/7/613 |
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