Study on Antioxidant Properties of Pueraria lobata Polysaccharides and Its Hypoglycemic Effect
Objective: To study the antioxidant activity of Pueraria lobata polysaccharide and its hypoglycemic effect. Method: The polysaccharides of Pueraria lobata were extracted by water extraction and alcohol precipitation method, and the polysaccharide content and the antioxidant capacity of Pueraria loba...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
The editorial department of Science and Technology of Food Industry
2022-03-01
|
| Series: | Shipin gongye ke-ji |
| Subjects: | |
| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021070357 |
| _version_ | 1798017464580177920 |
|---|---|
| author | Qiudan WANG Kaidi ZHAO Changqing LIN |
| author_facet | Qiudan WANG Kaidi ZHAO Changqing LIN |
| author_sort | Qiudan WANG |
| collection | DOAJ |
| description | Objective: To study the antioxidant activity of Pueraria lobata polysaccharide and its hypoglycemic effect. Method: The polysaccharides of Pueraria lobata were extracted by water extraction and alcohol precipitation method, and the polysaccharide content and the antioxidant capacity of Pueraria lobata polysaccharides were determined. Rats were intraperitoneally injected with streptozotocin (STZ) to establish a type 1 diabetes model (T1DM), which was divided into normal group (distilled water 100 mg/kg), model group (distilled water 100 mg/kg), high-dose Pueraria lobata polysaccharide group (100 mg/kg), Pueraria lobata polysaccharide low-dose group (50 mg/kg), positive group (metformin 100 mg/kg), the rats' body weight and fasting blood glucose (FBG) were measured regularly for 8 weeks. In the last week, an oral glucose tolerance (OGTT) test was performed, and rat serum and liver were taken to determine the changes in lipid metabolism indexes and related oxidases in rats. Results: The content of Pueraria lobata polysaccharide was 87.80%, and the scavenging abilities of DPPH·, ABTS+·, ·OH, and PTIO were 90.2%, 83.3%, 81.3%, and 89.0% at a concentration of 1000 μg/mL, respectively. Compared with the model group, Pueraria lobata polysaccharide could effectively alleviate the weight loss of T1DM rats, significantly reduced their FBG (P<0.05), and significantly increased the level of OGTT (P<0.05). After high-dose treatment with Pueraria lobata polysaccharide, TC, TG, the levels of LDL-C and MDA decreased significantly, and the levels of HDL-C, SOD, GSH and CAT increased significantly (P<0.05). All indicators were dose-dependent. Conclusion: Pueraria lobata polysaccharide has good antioxidant properties and can reduce blood sugar by improving lipid metabolism and oxidative stress in T1DM rats. |
| first_indexed | 2024-04-11T16:07:38Z |
| format | Article |
| id | doaj.art-a5f238e0c82742e38113b62908ad8beb |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-11T16:07:38Z |
| publishDate | 2022-03-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-a5f238e0c82742e38113b62908ad8beb2022-12-22T04:14:47ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062022-03-0143538138810.13386/j.issn1002-0306.20210703572021070357-5Study on Antioxidant Properties of Pueraria lobata Polysaccharides and Its Hypoglycemic EffectQiudan WANG0Kaidi ZHAO1Changqing LIN2Department of Traditional Chinese Medicine, School of Medicine, Yanbian University, Yanji 133000, ChinaDepartment of Traditional Chinese Medicine, School of Medicine, Yanbian University, Yanji 133000, ChinaDepartment of Traditional Chinese Medicine, School of Medicine, Yanbian University, Yanji 133000, ChinaObjective: To study the antioxidant activity of Pueraria lobata polysaccharide and its hypoglycemic effect. Method: The polysaccharides of Pueraria lobata were extracted by water extraction and alcohol precipitation method, and the polysaccharide content and the antioxidant capacity of Pueraria lobata polysaccharides were determined. Rats were intraperitoneally injected with streptozotocin (STZ) to establish a type 1 diabetes model (T1DM), which was divided into normal group (distilled water 100 mg/kg), model group (distilled water 100 mg/kg), high-dose Pueraria lobata polysaccharide group (100 mg/kg), Pueraria lobata polysaccharide low-dose group (50 mg/kg), positive group (metformin 100 mg/kg), the rats' body weight and fasting blood glucose (FBG) were measured regularly for 8 weeks. In the last week, an oral glucose tolerance (OGTT) test was performed, and rat serum and liver were taken to determine the changes in lipid metabolism indexes and related oxidases in rats. Results: The content of Pueraria lobata polysaccharide was 87.80%, and the scavenging abilities of DPPH·, ABTS+·, ·OH, and PTIO were 90.2%, 83.3%, 81.3%, and 89.0% at a concentration of 1000 μg/mL, respectively. Compared with the model group, Pueraria lobata polysaccharide could effectively alleviate the weight loss of T1DM rats, significantly reduced their FBG (P<0.05), and significantly increased the level of OGTT (P<0.05). After high-dose treatment with Pueraria lobata polysaccharide, TC, TG, the levels of LDL-C and MDA decreased significantly, and the levels of HDL-C, SOD, GSH and CAT increased significantly (P<0.05). All indicators were dose-dependent. Conclusion: Pueraria lobata polysaccharide has good antioxidant properties and can reduce blood sugar by improving lipid metabolism and oxidative stress in T1DM rats.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021070357pueraria lobata polysaccharideantioxidanthypoglycemiclipid metabolismoxidative stress |
| spellingShingle | Qiudan WANG Kaidi ZHAO Changqing LIN Study on Antioxidant Properties of Pueraria lobata Polysaccharides and Its Hypoglycemic Effect Shipin gongye ke-ji pueraria lobata polysaccharide antioxidant hypoglycemic lipid metabolism oxidative stress |
| title | Study on Antioxidant Properties of Pueraria lobata Polysaccharides and Its Hypoglycemic Effect |
| title_full | Study on Antioxidant Properties of Pueraria lobata Polysaccharides and Its Hypoglycemic Effect |
| title_fullStr | Study on Antioxidant Properties of Pueraria lobata Polysaccharides and Its Hypoglycemic Effect |
| title_full_unstemmed | Study on Antioxidant Properties of Pueraria lobata Polysaccharides and Its Hypoglycemic Effect |
| title_short | Study on Antioxidant Properties of Pueraria lobata Polysaccharides and Its Hypoglycemic Effect |
| title_sort | study on antioxidant properties of pueraria lobata polysaccharides and its hypoglycemic effect |
| topic | pueraria lobata polysaccharide antioxidant hypoglycemic lipid metabolism oxidative stress |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021070357 |
| work_keys_str_mv | AT qiudanwang studyonantioxidantpropertiesofpuerarialobatapolysaccharidesanditshypoglycemiceffect AT kaidizhao studyonantioxidantpropertiesofpuerarialobatapolysaccharidesanditshypoglycemiceffect AT changqinglin studyonantioxidantpropertiesofpuerarialobatapolysaccharidesanditshypoglycemiceffect |