A Novel FRET Approach Quantifies the Interaction Strength of Peroxisomal Targeting Signals and Their Receptor in Living Cells

Measuring Förster–resonance–energy–transfer (FRET) efficiency allows the investigation of protein–protein interactions (PPI), but extracting quantitative measures of affinity necessitates highly advanced technical equipment or isolated proteins. We demonstrate the validity of a recently suggested no...

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Bibliographic Details
Main Authors: Bernhard Hochreiter, Cheng-Shoong Chong, Andreas Hartig, Sebastian Maurer-Stroh, Johannes Berger, Johannes A. Schmid, Markus Kunze
Format: Article
Language:English
Published: MDPI AG 2020-10-01
Series:Cells
Subjects:
Online Access:https://www.mdpi.com/2073-4409/9/11/2381