Using High-Content Screening to Generate Single-Cell Gene-Corrected Patient-Derived iPS Clones Reveals Excess Alpha-Synuclein with Familial Parkinson’s Disease Point Mutation A30P

Using High-Content Screening to Generate Single-Cell Gene-Corrected Patient-Derived iPS Clones Reveals Excess Alpha-Synuclein with Familial Parkinson’s Disease Point Mutation A30P

The generation of isogenic induced pluripotent stem cell (iPSC) lines using CRISPR-Cas9 technology is a technically challenging, time-consuming process with variable efficiency. Here we use fluorescence-activated cell sorting (FACS) to sort biallelic CRISPR-Cas9 edited single-cell iPSC clones into h...

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Bibliographic Details
Main Authors: Peter Barbuti, Paul Antony, Bruno Santos, François Massart, Gérald Cruciani, Claire Dording, Jonathan Arias, Jens Schwamborn, Rejko Krüger
Format: Article
Language:English
Published: MDPI AG 2020-09-01
Series:Cells
Subjects:
CRISPR-Cas9
high-content screening (HCS)
fluorescent-activated cell sorting (FACS)
Parkinson’s disease (PD), patient-derived iPS
single-cell clones
isogenic cell lines
Online Access:https://www.mdpi.com/2073-4409/9/9/2065

Internet

https://www.mdpi.com/2073-4409/9/9/2065

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