Integrate thermostabilized fusion protein apocytochrome b562RIL and N-glycosylation mutations: A novel approach to heterologous expression of human UDP-glucuronosyltransferase (UGT) 2B7

Integrate thermostabilized fusion protein apocytochrome b562RIL and N-glycosylation mutations: A novel approach to heterologous expression of human UDP-glucuronosyltransferase (UGT) 2B7

Human UDP-glucuronosyltransferase (UGT) 2B7 is a crucial phase II metabolic enzyme that transfers glucuronic acid from UDP-glucuronic acid (UDPGA) to endobiotic and xenobiotic substrates. Biophysical and biochemical investigations of UGT2B7 are hampered by the challenge of the integral membrane prot...

Full description

Bibliographic Details
Main Authors: Jia Xue, Haitao Zhang, Su Zeng
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-08-01
Series:Frontiers in Pharmacology
Subjects:
UDP-glucuronosyltransferase 2B7
expression and purification
thermostabilization
mutations
enzymatic activity
surface plasmon resonance
Online Access:https://www.frontiersin.org/articles/10.3389/fphar.2022.965038/full

Internet

https://www.frontiersin.org/articles/10.3389/fphar.2022.965038/full

Similar Items