Two-Stage Alignment of FIB-SEM Images of Rock Samples

Focused Ion Beam Scanning Electron Microscopy (FIB-SEM) tomography provides a stack of images that represent serial slices of the sample. These images are displaced relatively to each other, and an alignment procedure is required. Traditional methods for alignment of a 3D image are based on a compar...

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Main Authors: Iryna Reimers, Ilia Safonov, Anton Kornilov, Ivan Yakimchuk
Format: Article
Language:English
Published: MDPI AG 2020-10-01
Series:Journal of Imaging
Subjects:
Online Access:https://www.mdpi.com/2313-433X/6/10/107
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author Iryna Reimers
Ilia Safonov
Anton Kornilov
Ivan Yakimchuk
author_facet Iryna Reimers
Ilia Safonov
Anton Kornilov
Ivan Yakimchuk
author_sort Iryna Reimers
collection DOAJ
description Focused Ion Beam Scanning Electron Microscopy (FIB-SEM) tomography provides a stack of images that represent serial slices of the sample. These images are displaced relatively to each other, and an alignment procedure is required. Traditional methods for alignment of a 3D image are based on a comparison of two adjacent slices. However, such algorithms are easily confused by anisotropy in the sample structure or even experiment geometry in the case of porous media. This may lead to significant distortions in the pore space geometry, if there are no stable fiducial marks in the frame. In this paper, we propose a new method, which meaningfully extends existing alignment procedures. Our technique allows the correction of random misalignments between slices and, at the same time, preserves the overall geometrical structure of the specimen. We consider displacements produced by existing alignment algorithms as a signal and decompose it into low and high-frequency components. Final transformations exclude slow variations and contain only high frequency variations that represent random shifts that need to be corrected. The proposed algorithm can operate with not only translations but also with arbitrary affine transformations. We demonstrate the performance of our approach on a synthetic dataset and two real FIB-SEM images of natural rock.
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spelling doaj.art-a672d2fb52f542d9b7031b5dcfba72652023-11-20T16:34:13ZengMDPI AGJournal of Imaging2313-433X2020-10-0161010710.3390/jimaging6100107Two-Stage Alignment of FIB-SEM Images of Rock SamplesIryna Reimers0Ilia Safonov1Anton Kornilov2Ivan Yakimchuk3Schlumberger Moscow Research Center, 125171 Moscow, RussiaSchlumberger Moscow Research Center, 125171 Moscow, RussiaSchlumberger Moscow Research Center, 125171 Moscow, RussiaSchlumberger Moscow Research Center, 125171 Moscow, RussiaFocused Ion Beam Scanning Electron Microscopy (FIB-SEM) tomography provides a stack of images that represent serial slices of the sample. These images are displaced relatively to each other, and an alignment procedure is required. Traditional methods for alignment of a 3D image are based on a comparison of two adjacent slices. However, such algorithms are easily confused by anisotropy in the sample structure or even experiment geometry in the case of porous media. This may lead to significant distortions in the pore space geometry, if there are no stable fiducial marks in the frame. In this paper, we propose a new method, which meaningfully extends existing alignment procedures. Our technique allows the correction of random misalignments between slices and, at the same time, preserves the overall geometrical structure of the specimen. We consider displacements produced by existing alignment algorithms as a signal and decompose it into low and high-frequency components. Final transformations exclude slow variations and contain only high frequency variations that represent random shifts that need to be corrected. The proposed algorithm can operate with not only translations but also with arbitrary affine transformations. We demonstrate the performance of our approach on a synthetic dataset and two real FIB-SEM images of natural rock.https://www.mdpi.com/2313-433X/6/10/107FIB-SEMalignmentdigital rockstack of imagesporous media
spellingShingle Iryna Reimers
Ilia Safonov
Anton Kornilov
Ivan Yakimchuk
Two-Stage Alignment of FIB-SEM Images of Rock Samples
Journal of Imaging
FIB-SEM
alignment
digital rock
stack of images
porous media
title Two-Stage Alignment of FIB-SEM Images of Rock Samples
title_full Two-Stage Alignment of FIB-SEM Images of Rock Samples
title_fullStr Two-Stage Alignment of FIB-SEM Images of Rock Samples
title_full_unstemmed Two-Stage Alignment of FIB-SEM Images of Rock Samples
title_short Two-Stage Alignment of FIB-SEM Images of Rock Samples
title_sort two stage alignment of fib sem images of rock samples
topic FIB-SEM
alignment
digital rock
stack of images
porous media
url https://www.mdpi.com/2313-433X/6/10/107
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AT iliasafonov twostagealignmentoffibsemimagesofrocksamples
AT antonkornilov twostagealignmentoffibsemimagesofrocksamples
AT ivanyakimchuk twostagealignmentoffibsemimagesofrocksamples