Development of ONT-cappable-seq to unravel the transcriptional landscape of Pseudomonas phages
RNA sequencing has become the method of choice to study the transcriptional landscape of phage-infected bacteria. However, short-read RNA sequencing approaches generally fail to capture the primary 5′ and 3′ boundaries of transcripts, confounding the discovery of key transcription initiation and ter...
Main Authors: | Leena Putzeys, Maarten Boon, Eveline-Marie Lammens, Konstantin Kuznedelov, Konstantin Severinov, Rob Lavigne |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2022-01-01
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Series: | Computational and Structural Biotechnology Journal |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2001037022001908 |
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