Chemical Genetic Validation of CSNK2 Substrates Using an Inhibitor-Resistant Mutant in Combination with Triple SILAC Quantitative Phosphoproteomics

Chemical Genetic Validation of CSNK2 Substrates Using an Inhibitor-Resistant Mutant in Combination with Triple SILAC Quantitative Phosphoproteomics

Casein Kinase 2 (CSNK2) is an extremely pleiotropic, ubiquitously expressed protein kinase involved in the regulation of numerous key biological processes. Mapping the CSNK2-dependent phosphoproteome is necessary for better characterization of its fundamental role in cellular signalling. While ATP-c...

Full description

Bibliographic Details
Main Authors:	Laszlo Gyenis, Daniel Menyhart, Edward S. Cruise, Kristina Jurcic, Scott E. Roffey, Darren B. Chai, Flaviu Trifoi, Sam R. Fess, Paul J. Desormeaux, Teresa Núñez de Villavicencio Díaz, Adam J. Rabalski, Stephanie A. Zukowski, Jacob P. Turowec, Paula Pittock, Gilles Lajoie, David W. Litchfield
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2022-06-01
Series:	Frontiers in Molecular Biosciences
Subjects:	protein kinase CK2 CSNK2 chemical genetics CX-4945 phosphoproteomics SILAC
Online Access:	https://www.frontiersin.org/articles/10.3389/fmolb.2022.909711/full

Similar Items

Phosphoproteomic mapping of CCR5 and ACKR2 signaling properties
by: Alessandro Vacchini, et al.
Published: (2022-11-01)

Phosphoproteomic Landscape of AML Cells Treated with the ATP-Competitive CK2 Inhibitor CX-4945
by: Mauro Rosales, et al.
Published: (2021-02-01)

Analysis of T4SS-induced signaling by H. pylori using quantitative phosphoproteomics
by: Frithjof eGlowinski, et al.
Published: (2014-07-01)

Phosphoproteome Study of Escherichia coli Devoid of Ser/Thr Kinase YeaG During the Metabolic Shift From Glucose to Malate
by: Abida Sultan, et al.
Published: (2021-04-01)

In Skeletal Muscle Fibers, Protein Kinase Subunit CSNK2A1/CK2α Is Required for Proper Muscle Homeostasis and Structure and Function of Neuromuscular Junctions
by: Mira Merholz, et al.
Published: (2022-12-01)

A SILAC-Based Approach Elicits the Proteomic Responses to Vancomycin-Associated Nephrotoxicity in Human Proximal Tubule Epithelial HK-2 Cells
by: Zhi-Ling Li, et al.
Published: (2016-01-01)

Unveiling the role of melatonin‐related gene CSNK1D in osteoclastogenesis and its implications for osteoporosis treatment
by: Jiewen Zhang, et al.
Published: (2025-02-01)

CK2 Regulation: Perspectives in 2021
by: Scott E. Roffey, et al.
Published: (2021-09-01)

Selecting Sample Preparation Workflows for Mass Spectrometry-Based Proteomic and Phosphoproteomic Analysis of Patient Samples with Acute Myeloid Leukemia
by: Maria Hernandez-Valladares, et al.
Published: (2016-08-01)

RETREG3/FAM134C phosphorylation by CSNK2 regulates reticulophagy during starvation
by: Giorgia Di Lorenzo, et al.
Published: (2022-12-01)

ALDR Enhanced Endothelial Injury in Hyperuricemia Screened using SILAC
by: Yang Zhang, et al.
Published: (2014-02-01)

CSNK2A2 promotes hepatocellular carcinoma progression through activation of NF-κB pathway
by: Shuang Yang, et al.
Published: (2023-09-01)

Involvement of casein kinase 1 epsilon/delta (Csnk1e/d) in the pathogenesis of familial Parkinson's disease caused by CHCHD2
by: Satoru Torii, et al.
Published: (2023-08-01)

CSNK2A1 Promotes Gastric Cancer Invasion Through the PI3K-Akt-mTOR Signaling Pathway
by: Jiang C, et al.
Published: (2019-12-01)

SILAC-based quantification of changes in protein tyrosine phosphorylation induced by Interleukin-2 (IL-2) and IL-15 in T-lymphocytes
by: Nerea Osinalde, et al.
Published: (2015-12-01)

CSNK2A1‐mediated phosphorylation of HMGA2 modulates cisplatin resistance in cervical cancer
by: Zhan Shi, et al.
Published: (2021-08-01)

Phenolization vs SILaC Laser: Which is More Successful in the Treatment of Pilonidal Sinus Disease?
by: Ömer Yalkın, et al.
Published: (2022-09-01)

Comprehensive landscape of gastric cancer-targeted therapy and identification of CSNK2A1 as a potential target
by: Liang Zhang, et al.
Published: (2024-08-01)

New RoxS sRNA Targets Identified in Bacillus subtilis by Pulsed SILAC
by: Delphine Allouche, et al.
Published: (2023-08-01)

De Novo CSNK2B Mutations in Five Cases of Poirier–Bienvenu Neurodevelopmental Syndrome
by: Qi Yang, et al.
Published: (2022-03-01)

Overexpression of NELFE contributes to gastric cancer progression via Wnt/β-catenin signaling-mediated activation of CSNK2B expression
by: Shijun Yu, et al.
Published: (2021-02-01)

SILAC-based quantitative proteomic analysis of Drosophila gastrula stage embryos mutant for fibroblast growth factor signalling
by: Hamze Beati, et al.
Published: (2020-10-01)

CSNK1E is involved in TGF-β1 induced epithelial mesenchymal transformationas and related to melanoma immune heterogeneity
by: Wangbing Hong, et al.
Published: (2025-01-01)

Age-dependent changes in protein incorporation into collagen-rich tissues of mice by in vivo pulsed SILAC labelling
by: Yoanna Ariosa-Morejon, et al.
Published: (2021-09-01)

Phylogenetic relationship based on flavonoids and its regions studies on selected Boesenbergia species in Sabah
by: Lam, Nyee Fan
Published: (2004)

Ecological studies on the nepenthes species along Kaingaran Trail of Mount Trus Madi, Sabah, Malaysia
by: Alviana Damit
Published: (2019)

Splicing Interruption by Intron Variants in CSNK2B Causes Poirier–Bienvenu Neurodevelopmental Syndrome: A Focus on Genotype–Phenotype Correlations
by: Wen Zhang, et al.
Published: (2022-06-01)

Measuring pathway database coverage of the phosphoproteome
by: Hannah Huckstep, et al.
Published: (2021-05-01)

Coupling of Cell Surface Biotinylation and SILAC-Based Quantitative Proteomics Identified Myoferlin as a Potential Therapeutic Target for Nasopharyngeal Carcinoma Metastasis
by: Maoyu Li, et al.
Published: (2021-06-01)

Synthesis of 5-Benzylamino and 5-Alkylamino-Substituted Pyrimido[4,5-c]quinoline Derivatives as CSNK2A Inhibitors with Antiviral Activity
by: Kesatebrhan Haile Asressu, et al.
Published: (2024-02-01)

SILAC-based quantitative proteomics using mass spectrometry quantifies endoplasmic reticulum stress in whole HeLa cells
by: Daniel N. Itzhak, et al.
Published: (2019-11-01)

CK2α/CSNK2A1 Induces Resistance to Doxorubicin through SIRT6-Mediated Activation of the DNA Damage Repair Pathway
by: Usama Khamis Hussein, et al.
Published: (2021-07-01)

Upregulation of CSNK1A1 induced by ITGB5 confers to hepatocellular carcinoma resistance to sorafenib in vivo by disrupting the EPS15/EGFR complex
by: Li Gu, et al.
Published: (2023-06-01)

Loss of Protein Kinase Csnk2b/CK2β at Neuromuscular Junctions Affects Morphology and Dynamics of Aggregated Nicotinic Acetylcholine Receptors, Neuromuscular Transmission, and Synaptic Gene Expression
by: Nane Eiber, et al.
Published: (2019-08-01)

Quantitative Analysis of Differential Proteome Expression in Epithelial-to-Mesenchymal Transition of Bladder Epithelial Cells Using SILAC Method
by: Ganglong Yang, et al.
Published: (2016-01-01)

Pulsed SILAC-based proteomic analysis unveils hypoxia- and serum starvation-induced de novo protein synthesis with PHD finger protein 14 (PHF14) as a hypoxia sensitive epigenetic regulator in cell cycle progression
by: Park, Jung Eun, et al.
Published: (2020)

Circadian gene CSNK1D promoted the progression of hepatocellular carcinoma by activating Wnt/β-catenin pathway via stabilizing Dishevelled Segment Polarity Protein 3
by: Mengqi Zhu, et al.
Published: (2022-12-01)

Insights into the Lysine Acetylome of the Haloarchaeon <i>Haloferax volcanii</i> during Oxidative Stress by Quantitative SILAC-Based Proteomics
by: Ricardo L. Couto-Rodríguez, et al.
Published: (2023-06-01)

Phosphoproteome Discovery in Human Biological Fluids
by: Francesco Giorgianni, et al.
Published: (2016-12-01)

Dynamic proteomic and phosphoproteomic atlas of corticostriatal axons in neurodevelopment
by: Vasin Dumrongprechachan, et al.
Published: (2022-10-01)