Construction of Mycobacterium tuberculosis cdd knockout and evaluation of invasion and growth in macrophages
Cytidine deaminase (MtCDA), encoded by cdd gene (Rv3315c), is the only enzyme identified in nucleotide biosynthesis pathway of Mycobacterium tuberculosis that is able to recycle cytidine and deoxycytidine. An M. tuberculosis knockout strain for cdd gene was obtained by allelic replacement. Evaluatio...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762017001100785&lng=en&tlng=en |
| _version_ | 1797756587450826752 |
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| author | Anne Drumond Villela Valnês S Rodrigues-Junior Antônio Frederico Michel Pinto Virgínia Carla de Almeida Falcão Zilpa Adriana Sánchez-Quitian Paula Eichler Cristiano Valim Bizarro Luiz Augusto Basso Diógenes Santiago Santos |
| author_facet | Anne Drumond Villela Valnês S Rodrigues-Junior Antônio Frederico Michel Pinto Virgínia Carla de Almeida Falcão Zilpa Adriana Sánchez-Quitian Paula Eichler Cristiano Valim Bizarro Luiz Augusto Basso Diógenes Santiago Santos |
| author_sort | Anne Drumond Villela |
| collection | DOAJ |
| description | Cytidine deaminase (MtCDA), encoded by cdd gene (Rv3315c), is the only enzyme identified in nucleotide biosynthesis pathway of Mycobacterium tuberculosis that is able to recycle cytidine and deoxycytidine. An M. tuberculosis knockout strain for cdd gene was obtained by allelic replacement. Evaluation of mRNA expression validated cdd deletion and showed the absence of polar effect. MudPIT LC-MS/MS data indicated thymidine phosphorylase expression was decreased in knockout and complemented strains. The cdd disruption does not affect M. tuberculosis growth both in Mid- dlebrook 7H9 and in RAW 264.7 cells, which indicates that cdd is not important for macrophage invasion and virulence. |
| first_indexed | 2024-03-12T18:03:43Z |
| format | Article |
| id | doaj.art-a68ff91c0efe4a87ba254ba61e4341e6 |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:03:43Z |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-a68ff91c0efe4a87ba254ba61e4341e62023-08-02T09:32:25ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80601121178578910.1590/0074-02760170105S0074-02762017001100785Construction of Mycobacterium tuberculosis cdd knockout and evaluation of invasion and growth in macrophagesAnne Drumond VillelaValnês S Rodrigues-JuniorAntônio Frederico Michel PintoVirgínia Carla de Almeida FalcãoZilpa Adriana Sánchez-QuitianPaula EichlerCristiano Valim BizarroLuiz Augusto BassoDiógenes Santiago SantosCytidine deaminase (MtCDA), encoded by cdd gene (Rv3315c), is the only enzyme identified in nucleotide biosynthesis pathway of Mycobacterium tuberculosis that is able to recycle cytidine and deoxycytidine. An M. tuberculosis knockout strain for cdd gene was obtained by allelic replacement. Evaluation of mRNA expression validated cdd deletion and showed the absence of polar effect. MudPIT LC-MS/MS data indicated thymidine phosphorylase expression was decreased in knockout and complemented strains. The cdd disruption does not affect M. tuberculosis growth both in Mid- dlebrook 7H9 and in RAW 264.7 cells, which indicates that cdd is not important for macrophage invasion and virulence.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762017001100785&lng=en&tlng=enMycobacterium tuberculosiscdd genecytidine deaminase |
| spellingShingle | Anne Drumond Villela Valnês S Rodrigues-Junior Antônio Frederico Michel Pinto Virgínia Carla de Almeida Falcão Zilpa Adriana Sánchez-Quitian Paula Eichler Cristiano Valim Bizarro Luiz Augusto Basso Diógenes Santiago Santos Construction of Mycobacterium tuberculosis cdd knockout and evaluation of invasion and growth in macrophages Memorias do Instituto Oswaldo Cruz Mycobacterium tuberculosis cdd gene cytidine deaminase |
| title | Construction of Mycobacterium tuberculosis cdd knockout and evaluation of invasion and growth in macrophages |
| title_full | Construction of Mycobacterium tuberculosis cdd knockout and evaluation of invasion and growth in macrophages |
| title_fullStr | Construction of Mycobacterium tuberculosis cdd knockout and evaluation of invasion and growth in macrophages |
| title_full_unstemmed | Construction of Mycobacterium tuberculosis cdd knockout and evaluation of invasion and growth in macrophages |
| title_short | Construction of Mycobacterium tuberculosis cdd knockout and evaluation of invasion and growth in macrophages |
| title_sort | construction of mycobacterium tuberculosis cdd knockout and evaluation of invasion and growth in macrophages |
| topic | Mycobacterium tuberculosis cdd gene cytidine deaminase |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762017001100785&lng=en&tlng=en |
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