Validated DBS method for filgotinib quantitation in rat dried blood spots and its application to a pharmacokinetic study in rats
Filgotinib is a selective JAK1 (Janus kinase) inhibitor, showed efficacy in patients suffering from moderate-to-severe rheumatoid arthritis. In this paper, we present the data on the development and validation of a sensitive, selective and high-throughput LC-MS/MS (liquid chromatography with tandem...
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| Format: | Article |
| Language: | English |
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International Association of Physical Chemists (IAPC)
2020-06-01
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| Series: | ADMET and DMPK |
| Subjects: | |
| Online Access: | http://pub.iapchem.org/ojs/index.php/admet/article/view/796 |
| _version_ | 1819150773633679360 |
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| author | Abhishek Dixit Vinay Kiran Bhavesh Babulal Gabani Ramesh Mullangi |
| author_facet | Abhishek Dixit Vinay Kiran Bhavesh Babulal Gabani Ramesh Mullangi |
| author_sort | Abhishek Dixit |
| collection | DOAJ |
| description | Filgotinib is a selective JAK1 (Janus kinase) inhibitor, showed efficacy in patients suffering from moderate-to-severe rheumatoid arthritis. In this paper, we present the data on the development and validation of a sensitive, selective and high-throughput LC-MS/MS (liquid chromatography with tandem mass spectrometry) method for the quantitation of filgotinib from rat dried blood spot (DBS) cards. To the DBS disc cards, 0.2 % formic acid enriched with internal standard (IS) was added and sonicated. Thereafter the extraction of filgotinib and the IS (tofacitinib) was accomplished using ethyl acetate as an extraction solvent. The resolution of filgotinib and the IS was achieved on a Gemini C18 column with an isocratic mobile phase, which is a mixture of 0.2 % formic acid:acetonitrile (20:80, v/v) at a flow-rate of 0.9 mL/min. The total run time was 2.90 min and the retention time of filgotinib and the IS was ~1.31 and 0.89 min, respectively. Filgotinib and the IS were analyzed using positive ion scan mode and parent-daughter mass to charge ion (m/z) transition of 426.3®291.3 and m/z 313.2®149.2, respectively, for quantitation. The calibration range was 1.37-1937 ng/mL. No matrix effect and carry over were observed. All the validation parameters met the acceptance criteria. The validated method has been applied to a pharmacokinetic study in rats. A good correlation between DBS and plasma concentrations for filgotinib was observed. |
| first_indexed | 2024-12-22T14:22:50Z |
| format | Article |
| id | doaj.art-a69742d7530944ae98f92ce57735228b |
| institution | Directory Open Access Journal |
| issn | 1848-7718 |
| language | English |
| last_indexed | 2024-12-22T14:22:50Z |
| publishDate | 2020-06-01 |
| publisher | International Association of Physical Chemists (IAPC) |
| record_format | Article |
| series | ADMET and DMPK |
| spelling | doaj.art-a69742d7530944ae98f92ce57735228b2022-12-21T18:22:56ZengInternational Association of Physical Chemists (IAPC)ADMET and DMPK1848-77182020-06-018213914810.5599/admet.796426Validated DBS method for filgotinib quantitation in rat dried blood spots and its application to a pharmacokinetic study in ratsAbhishek DixitVinay KiranBhavesh Babulal GabaniRamesh MullangiFilgotinib is a selective JAK1 (Janus kinase) inhibitor, showed efficacy in patients suffering from moderate-to-severe rheumatoid arthritis. In this paper, we present the data on the development and validation of a sensitive, selective and high-throughput LC-MS/MS (liquid chromatography with tandem mass spectrometry) method for the quantitation of filgotinib from rat dried blood spot (DBS) cards. To the DBS disc cards, 0.2 % formic acid enriched with internal standard (IS) was added and sonicated. Thereafter the extraction of filgotinib and the IS (tofacitinib) was accomplished using ethyl acetate as an extraction solvent. The resolution of filgotinib and the IS was achieved on a Gemini C18 column with an isocratic mobile phase, which is a mixture of 0.2 % formic acid:acetonitrile (20:80, v/v) at a flow-rate of 0.9 mL/min. The total run time was 2.90 min and the retention time of filgotinib and the IS was ~1.31 and 0.89 min, respectively. Filgotinib and the IS were analyzed using positive ion scan mode and parent-daughter mass to charge ion (m/z) transition of 426.3®291.3 and m/z 313.2®149.2, respectively, for quantitation. The calibration range was 1.37-1937 ng/mL. No matrix effect and carry over were observed. All the validation parameters met the acceptance criteria. The validated method has been applied to a pharmacokinetic study in rats. A good correlation between DBS and plasma concentrations for filgotinib was observed.http://pub.iapchem.org/ojs/index.php/admet/article/view/796filgotiniblc-ms/msmethod validationrat blooddbspharmacokinetics |
| spellingShingle | Abhishek Dixit Vinay Kiran Bhavesh Babulal Gabani Ramesh Mullangi Validated DBS method for filgotinib quantitation in rat dried blood spots and its application to a pharmacokinetic study in rats ADMET and DMPK filgotinib lc-ms/ms method validation rat blood dbs pharmacokinetics |
| title | Validated DBS method for filgotinib quantitation in rat dried blood spots and its application to a pharmacokinetic study in rats |
| title_full | Validated DBS method for filgotinib quantitation in rat dried blood spots and its application to a pharmacokinetic study in rats |
| title_fullStr | Validated DBS method for filgotinib quantitation in rat dried blood spots and its application to a pharmacokinetic study in rats |
| title_full_unstemmed | Validated DBS method for filgotinib quantitation in rat dried blood spots and its application to a pharmacokinetic study in rats |
| title_short | Validated DBS method for filgotinib quantitation in rat dried blood spots and its application to a pharmacokinetic study in rats |
| title_sort | validated dbs method for filgotinib quantitation in rat dried blood spots and its application to a pharmacokinetic study in rats |
| topic | filgotinib lc-ms/ms method validation rat blood dbs pharmacokinetics |
| url | http://pub.iapchem.org/ojs/index.php/admet/article/view/796 |
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