Drosophila FGFR/Htl signaling shapes embryonic glia to phagocytose apoptotic neurons
Abstract Glial phagocytosis of apoptotic neurons is crucial for development and proper function of the central nervous system. Relying on transmembrane receptors located on their protrusions, phagocytic glia recognize and engulf apoptotic debris. Like vertebrate microglia, Drosophila phagocytic glia...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Nature Publishing Group
2023-03-01
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| Series: | Cell Death Discovery |
| Online Access: | https://doi.org/10.1038/s41420-023-01382-5 |
| _version_ | 1797865553705041920 |
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| author | Malak Ayoub Li-mor David Boris Shklyar Ketty Hakim-Mishnaevski Estee Kurant |
| author_facet | Malak Ayoub Li-mor David Boris Shklyar Ketty Hakim-Mishnaevski Estee Kurant |
| author_sort | Malak Ayoub |
| collection | DOAJ |
| description | Abstract Glial phagocytosis of apoptotic neurons is crucial for development and proper function of the central nervous system. Relying on transmembrane receptors located on their protrusions, phagocytic glia recognize and engulf apoptotic debris. Like vertebrate microglia, Drosophila phagocytic glial cells form an elaborate network in the developing brain to reach and remove apoptotic neurons. However, the mechanisms controlling creation of the branched morphology of these glial cells critical for their phagocytic ability remain unknown. Here, we demonstrate that during early embryogenesis, the Drosophila fibroblast growth factor receptor (FGFR) Heartless (Htl) and its ligand Pyramus are essential in glial cells for the formation of glial extensions, the presence of which strongly affects glial phagocytosis of apoptotic neurons during later stages of embryonic development. Reduction in Htl pathway activity results in shorter lengths and lower complexity of glial branches, thereby disrupting the glial network. Our work thus illuminates the important role Htl signaling plays in glial subcellular morphogenesis and in establishing glial phagocytic ability. |
| first_indexed | 2024-04-09T23:11:02Z |
| format | Article |
| id | doaj.art-a6d14ebb493146dbb5bfa140324f549a |
| institution | Directory Open Access Journal |
| issn | 2058-7716 |
| language | English |
| last_indexed | 2024-04-09T23:11:02Z |
| publishDate | 2023-03-01 |
| publisher | Nature Publishing Group |
| record_format | Article |
| series | Cell Death Discovery |
| spelling | doaj.art-a6d14ebb493146dbb5bfa140324f549a2023-03-22T10:25:48ZengNature Publishing GroupCell Death Discovery2058-77162023-03-019111010.1038/s41420-023-01382-5Drosophila FGFR/Htl signaling shapes embryonic glia to phagocytose apoptotic neuronsMalak Ayoub0Li-mor David1Boris Shklyar2Ketty Hakim-Mishnaevski3Estee Kurant4Department of Human Biology, Faculty of Natural Sciences, University of HaifaDepartment of Human Biology, Faculty of Natural Sciences, University of HaifaBioimaging Unit, Faculty of Natural Sciences, University of HaifaDepartment of Human Biology, Faculty of Natural Sciences, University of HaifaDepartment of Human Biology, Faculty of Natural Sciences, University of HaifaAbstract Glial phagocytosis of apoptotic neurons is crucial for development and proper function of the central nervous system. Relying on transmembrane receptors located on their protrusions, phagocytic glia recognize and engulf apoptotic debris. Like vertebrate microglia, Drosophila phagocytic glial cells form an elaborate network in the developing brain to reach and remove apoptotic neurons. However, the mechanisms controlling creation of the branched morphology of these glial cells critical for their phagocytic ability remain unknown. Here, we demonstrate that during early embryogenesis, the Drosophila fibroblast growth factor receptor (FGFR) Heartless (Htl) and its ligand Pyramus are essential in glial cells for the formation of glial extensions, the presence of which strongly affects glial phagocytosis of apoptotic neurons during later stages of embryonic development. Reduction in Htl pathway activity results in shorter lengths and lower complexity of glial branches, thereby disrupting the glial network. Our work thus illuminates the important role Htl signaling plays in glial subcellular morphogenesis and in establishing glial phagocytic ability.https://doi.org/10.1038/s41420-023-01382-5 |
| spellingShingle | Malak Ayoub Li-mor David Boris Shklyar Ketty Hakim-Mishnaevski Estee Kurant Drosophila FGFR/Htl signaling shapes embryonic glia to phagocytose apoptotic neurons Cell Death Discovery |
| title | Drosophila FGFR/Htl signaling shapes embryonic glia to phagocytose apoptotic neurons |
| title_full | Drosophila FGFR/Htl signaling shapes embryonic glia to phagocytose apoptotic neurons |
| title_fullStr | Drosophila FGFR/Htl signaling shapes embryonic glia to phagocytose apoptotic neurons |
| title_full_unstemmed | Drosophila FGFR/Htl signaling shapes embryonic glia to phagocytose apoptotic neurons |
| title_short | Drosophila FGFR/Htl signaling shapes embryonic glia to phagocytose apoptotic neurons |
| title_sort | drosophila fgfr htl signaling shapes embryonic glia to phagocytose apoptotic neurons |
| url | https://doi.org/10.1038/s41420-023-01382-5 |
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