Integrating Continuous Transepithelial Flux Measurements into an Ussing Chamber Set-Up

Integrating Continuous Transepithelial Flux Measurements into an Ussing Chamber Set-Up

Fluorescently labelled compounds are often employed to study the paracellular properties of epithelia. For flux measurements, these compounds are added to the donor compartment and samples collected from the acceptor compartment at regular intervals. However, this method fails to detect rapid change...

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Bibliographic Details
Main Authors: Çlirim Alija, Lukas Knobe, Ioanna Pouyiourou, Mikio Furuse, Rita Rosenthal, Dorothee Günzel
Format: Article
Language:English
Published: MDPI AG 2024-02-01
Series:International Journal of Molecular Sciences
Subjects:
transepithelial flux
paracellular fluorescent marker
Ussing chamber
automatized flux measurement
tight junction
osmotic stress
Online Access:https://www.mdpi.com/1422-0067/25/4/2252

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https://www.mdpi.com/1422-0067/25/4/2252

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