Pectins, hemicellulose and lignocellulose profiles vary in leaves among different aromatic Vanilla species (Orchidaceae)
The alcohol-insoluble residue in Vanilla dressleri, V. odorata, V. planifolia and V. pompona mature leaves was fractionated into water-, oxalate-, HCl- and NaOH-soluble pectins, hemicellulose and lignocellulose. Neutral sugars and uronic acids of each fraction were determined by high-performance ani...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Carbohydrate Polymer Technologies and Applications |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666893923000105 |
| _version_ | 1797801723718270976 |
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| author | Jorge Warner Tobias Pöhnl Christof B. Steingass Diego Bogarín Reinhold Carle Víctor M. Jiménez |
| author_facet | Jorge Warner Tobias Pöhnl Christof B. Steingass Diego Bogarín Reinhold Carle Víctor M. Jiménez |
| author_sort | Jorge Warner |
| collection | DOAJ |
| description | The alcohol-insoluble residue in Vanilla dressleri, V. odorata, V. planifolia and V. pompona mature leaves was fractionated into water-, oxalate-, HCl- and NaOH-soluble pectins, hemicellulose and lignocellulose. Neutral sugars and uronic acids of each fraction were determined by high-performance anion exchange chromatography-pulsed amperometric detection. V. dressleri differentiated from the other three species based on the higher amounts of the HCl-soluble pectin fraction and the amount of glucose exceeding 5–7 times the values found in the NaOH-soluble pectin fraction in the other species, as well as a cellulose/hemicellulose ratio of ca. 2/3. Analogously, V. planifolia could also be distinguished by its higher cellulose/hemicellulose ratio of ca. 7. In addition to taxonomical implications, these results might be instrumental for defining adequate conditions for cell wall digestion aiming at protoplast isolation and appropriate media composition for cell wall regeneration during protoplast culture, which has been elusive in the Orchidaceae. |
| first_indexed | 2024-03-13T04:54:50Z |
| format | Article |
| id | doaj.art-a705f62643254134a7b545374257b557 |
| institution | Directory Open Access Journal |
| issn | 2666-8939 |
| language | English |
| last_indexed | 2024-03-13T04:54:50Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Carbohydrate Polymer Technologies and Applications |
| spelling | doaj.art-a705f62643254134a7b545374257b5572023-06-18T05:03:42ZengElsevierCarbohydrate Polymer Technologies and Applications2666-89392023-06-015100289Pectins, hemicellulose and lignocellulose profiles vary in leaves among different aromatic Vanilla species (Orchidaceae)Jorge Warner0Tobias Pöhnl1Christof B. Steingass2Diego Bogarín3Reinhold Carle4Víctor M. Jiménez5Jardín Botánico Lankester, Universidad de Costa Rica, Cartago 302-7050, Costa RicaChair of Plant Foodstuff Technology and Analysis, University of Hohenheim, Stuttgart 70599, Federal Republic of GermanyChair of Plant Foodstuff Technology and Analysis, University of Hohenheim, Stuttgart 70599, Federal Republic of GermanyJardín Botánico Lankester, Universidad de Costa Rica, Cartago 302-7050, Costa RicaChair of Plant Foodstuff Technology and Analysis, University of Hohenheim, Stuttgart 70599, Federal Republic of GermanyCIGRAS/IAA, Universidad de Costa Rica, San Pedro 11501-2060, Costa Rica; Corresponding author.The alcohol-insoluble residue in Vanilla dressleri, V. odorata, V. planifolia and V. pompona mature leaves was fractionated into water-, oxalate-, HCl- and NaOH-soluble pectins, hemicellulose and lignocellulose. Neutral sugars and uronic acids of each fraction were determined by high-performance anion exchange chromatography-pulsed amperometric detection. V. dressleri differentiated from the other three species based on the higher amounts of the HCl-soluble pectin fraction and the amount of glucose exceeding 5–7 times the values found in the NaOH-soluble pectin fraction in the other species, as well as a cellulose/hemicellulose ratio of ca. 2/3. Analogously, V. planifolia could also be distinguished by its higher cellulose/hemicellulose ratio of ca. 7. In addition to taxonomical implications, these results might be instrumental for defining adequate conditions for cell wall digestion aiming at protoplast isolation and appropriate media composition for cell wall regeneration during protoplast culture, which has been elusive in the Orchidaceae.http://www.sciencedirect.com/science/article/pii/S2666893923000105Alcohol-insoluble residuesCell wallNeutral sugarsPolysaccharidesUronic acidsVanilla |
| spellingShingle | Jorge Warner Tobias Pöhnl Christof B. Steingass Diego Bogarín Reinhold Carle Víctor M. Jiménez Pectins, hemicellulose and lignocellulose profiles vary in leaves among different aromatic Vanilla species (Orchidaceae) Carbohydrate Polymer Technologies and Applications Alcohol-insoluble residues Cell wall Neutral sugars Polysaccharides Uronic acids Vanilla |
| title | Pectins, hemicellulose and lignocellulose profiles vary in leaves among different aromatic Vanilla species (Orchidaceae) |
| title_full | Pectins, hemicellulose and lignocellulose profiles vary in leaves among different aromatic Vanilla species (Orchidaceae) |
| title_fullStr | Pectins, hemicellulose and lignocellulose profiles vary in leaves among different aromatic Vanilla species (Orchidaceae) |
| title_full_unstemmed | Pectins, hemicellulose and lignocellulose profiles vary in leaves among different aromatic Vanilla species (Orchidaceae) |
| title_short | Pectins, hemicellulose and lignocellulose profiles vary in leaves among different aromatic Vanilla species (Orchidaceae) |
| title_sort | pectins hemicellulose and lignocellulose profiles vary in leaves among different aromatic vanilla species orchidaceae |
| topic | Alcohol-insoluble residues Cell wall Neutral sugars Polysaccharides Uronic acids Vanilla |
| url | http://www.sciencedirect.com/science/article/pii/S2666893923000105 |
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