CRISPR-PCS Protocol for Chromosome Splitting and Splitting Event Detection in Saccharomyces cerevisiae

Chromosome engineering is an important technology with applications in basic biology and biotechnology. Chromosome splitting technology called PCS (PCR-mediated Chromosome Splitting) has already been developed as a fundamental chromosome engineering technology in the budding yeast. However, the spli...

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Bibliographic Details
Main Authors: Yu Sasano, Satoshi Harashima
Format: Article
Language:English
Published: Bio-protocol LLC 2017-05-01
Series:Bio-Protocol
Online Access:https://bio-protocol.org/en/bpdetail?id=2306&type=0