Underestimation of SARS-CoV-2 in wastewater due to single or double mutations in the N1 qPCR probe binding region

Underestimation of SARS-CoV-2 in wastewater due to single or double mutations in the N1 qPCR probe binding region

Wastewater surveillance using RT-qPCR has now been widely adopted to track circulating levels of SARS-CoV-2 virus in many sewersheds. The CDC qPCR assays targeting two regions (N1 and N2) within the N gene are commonly used, but a discrepancy between the two biomarkers has been noticed by independen...

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Bibliographic Details
Main Authors: Jianxian Sun, Minqing Ivy Yang, Jiaxi Peng, Ismail Khan, Jhoselyn Jaramillo Lopez, Ronny Chan, Elizabeth A. Edwards, Hui Peng
Format: Article
Language:English
Published: Elsevier 2024-01-01
Series:Water Research X
Subjects:
SARS-CoV-2
Wastewater surveillance
RT-qPCR
Mutations
N1 dropout
Online Access:http://www.sciencedirect.com/science/article/pii/S2589914724000112

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http://www.sciencedirect.com/science/article/pii/S2589914724000112

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