Underestimation of SARS-CoV-2 in wastewater due to single or double mutations in the N1 qPCR probe binding region
Wastewater surveillance using RT-qPCR has now been widely adopted to track circulating levels of SARS-CoV-2 virus in many sewersheds. The CDC qPCR assays targeting two regions (N1 and N2) within the N gene are commonly used, but a discrepancy between the two biomarkers has been noticed by independen...
Main Authors: | Jianxian Sun, Minqing Ivy Yang, Jiaxi Peng, Ismail Khan, Jhoselyn Jaramillo Lopez, Ronny Chan, Elizabeth A. Edwards, Hui Peng |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2024-01-01
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Series: | Water Research X |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2589914724000112 |
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