The effects of different night-time temperatures and cultivation durations on the polyphenolic contents of lettuce: Application of principal component analysis
The present study was conducted to characterize the polyphenolic contents of lettuce leaves grown under different night-time temperatures (4, 12, and 20 °C) and cultivation durations (5, 15, and 20 days) using high performance liquid chromatography-tandem mass spectrometry (LC/MS/MS). The assay meth...
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| Format: | Article |
| Language: | English |
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Elsevier
2015-05-01
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| Series: | Journal of Advanced Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2090123215000053 |
| _version_ | 1828409228745768960 |
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| author | Sung Woo Jeong Gon-Sup Kim Won Sup Lee Yun-Hi Kim Nam Jun Kang Jong Sung Jin Gye Min Lee Soo Taek Kim A.M. Abd El-Aty Jae-Han Shim Sung Chul Shin |
| author_facet | Sung Woo Jeong Gon-Sup Kim Won Sup Lee Yun-Hi Kim Nam Jun Kang Jong Sung Jin Gye Min Lee Soo Taek Kim A.M. Abd El-Aty Jae-Han Shim Sung Chul Shin |
| author_sort | Sung Woo Jeong |
| collection | DOAJ |
| description | The present study was conducted to characterize the polyphenolic contents of lettuce leaves grown under different night-time temperatures (4, 12, and 20 °C) and cultivation durations (5, 15, and 20 days) using high performance liquid chromatography-tandem mass spectrometry (LC/MS/MS). The assay method was validated based on specificity, linearity, accuracy, precision, and the performance limit. The total polyphenolic contents were highest (2462.6 mg/kg) after transplantation at a night temperature of 20 °C on day 20 and lowest (1132.7 mg/kg) at the same temperature on day 5. Quantification and principal component analysis showed that the relative contents of quercetin and kaempferol were markedly higher during the early stage of cultivation (day 5) than those of day 15 and 20, and that night-time temperatures of 12 and 20 °C on day 20 were favorable for producing polyphenol-rich lettuce containing caffeic acid. In conclusion, a synergistic effect between high night-time temperatures (12 and 20 °C) and cultivation duration (20 days) produced lettuce rich in polyphenols compared to that at low temperature (4 °C). |
| first_indexed | 2024-12-10T11:52:38Z |
| format | Article |
| id | doaj.art-a75d172cad3c4be1b06ea58103b713b1 |
| institution | Directory Open Access Journal |
| issn | 2090-1232 2090-1224 |
| language | English |
| last_indexed | 2024-12-10T11:52:38Z |
| publishDate | 2015-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Advanced Research |
| spelling | doaj.art-a75d172cad3c4be1b06ea58103b713b12022-12-22T01:49:53ZengElsevierJournal of Advanced Research2090-12322090-12242015-05-016349349910.1016/j.jare.2015.01.004The effects of different night-time temperatures and cultivation durations on the polyphenolic contents of lettuce: Application of principal component analysisSung Woo Jeong0Gon-Sup Kim1Won Sup Lee2Yun-Hi Kim3Nam Jun Kang4Jong Sung Jin5Gye Min Lee6Soo Taek Kim7A.M. Abd El-Aty8Jae-Han Shim9Sung Chul Shin10Department of Chemistry and Research Institute of Life Science, Gyeongsang National University, Jinju 660−701, Republic of KoreaResearch Institute of Life Science and College of Veterinary Medicine, Gyeongsang National University, Jinju 660−701, Republic of KoreaDepartment of Internal Medicine, Institute of Health Sciences, Gyeongsang National University, Jinju 660−701, Republic of KoreaDepartment of Chemistry and Research Institute of Life Science, Gyeongsang National University, Jinju 660−701, Republic of KoreaDepartment of Horticulture and Research Institute of Life Science, Gyeongsang National University, Jinju 660−701, Republic of KoreaKorea Basic Science Institute Busan Centre, Division of High Technology Materials Research, Gangseo-gu, Busan 618-230, Republic of KoreaDepartment of Information Statistics, Research Institute of Natural Science, Gyeongsang National University, Jinju 660-701, Republic of KoreaDepartment of Information Statistics, Research Institute of Natural Science, Gyeongsang National University, Jinju 660-701, Republic of KoreaBiotechnology Research Institute, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 500-757, Republic of KoreaBiotechnology Research Institute, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 500-757, Republic of KoreaDepartment of Chemistry and Research Institute of Life Science, Gyeongsang National University, Jinju 660−701, Republic of KoreaThe present study was conducted to characterize the polyphenolic contents of lettuce leaves grown under different night-time temperatures (4, 12, and 20 °C) and cultivation durations (5, 15, and 20 days) using high performance liquid chromatography-tandem mass spectrometry (LC/MS/MS). The assay method was validated based on specificity, linearity, accuracy, precision, and the performance limit. The total polyphenolic contents were highest (2462.6 mg/kg) after transplantation at a night temperature of 20 °C on day 20 and lowest (1132.7 mg/kg) at the same temperature on day 5. Quantification and principal component analysis showed that the relative contents of quercetin and kaempferol were markedly higher during the early stage of cultivation (day 5) than those of day 15 and 20, and that night-time temperatures of 12 and 20 °C on day 20 were favorable for producing polyphenol-rich lettuce containing caffeic acid. In conclusion, a synergistic effect between high night-time temperatures (12 and 20 °C) and cultivation duration (20 days) produced lettuce rich in polyphenols compared to that at low temperature (4 °C).http://www.sciencedirect.com/science/article/pii/S2090123215000053Lactuca sativa L.Night growth temperaturePrincipal component analysisPolyphenolsCompositions |
| spellingShingle | Sung Woo Jeong Gon-Sup Kim Won Sup Lee Yun-Hi Kim Nam Jun Kang Jong Sung Jin Gye Min Lee Soo Taek Kim A.M. Abd El-Aty Jae-Han Shim Sung Chul Shin The effects of different night-time temperatures and cultivation durations on the polyphenolic contents of lettuce: Application of principal component analysis Journal of Advanced Research Lactuca sativa L. Night growth temperature Principal component analysis Polyphenols Compositions |
| title | The effects of different night-time temperatures and cultivation durations on the polyphenolic contents of lettuce: Application of principal component analysis |
| title_full | The effects of different night-time temperatures and cultivation durations on the polyphenolic contents of lettuce: Application of principal component analysis |
| title_fullStr | The effects of different night-time temperatures and cultivation durations on the polyphenolic contents of lettuce: Application of principal component analysis |
| title_full_unstemmed | The effects of different night-time temperatures and cultivation durations on the polyphenolic contents of lettuce: Application of principal component analysis |
| title_short | The effects of different night-time temperatures and cultivation durations on the polyphenolic contents of lettuce: Application of principal component analysis |
| title_sort | effects of different night time temperatures and cultivation durations on the polyphenolic contents of lettuce application of principal component analysis |
| topic | Lactuca sativa L. Night growth temperature Principal component analysis Polyphenols Compositions |
| url | http://www.sciencedirect.com/science/article/pii/S2090123215000053 |
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