Identification of seed coat phenolic compounds from differently colored pea varieties and characterization of their antioxidant activity
The phenolic composition of seed coats in four differently colored pea varieties (Pisum sativum L.) was investigated using UHPLC-LTQ OrbiTrap MS. The obtained findings revealed that the seed coats of the examined pea genotypes possess a unique phenolic composition compared to previously...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
University of Belgrade, University of Novi Sad
2015-01-01
|
| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2015/0354-46641500042S.pdf |
| Summary: | The phenolic composition of seed coats in four differently colored pea
varieties (Pisum sativum L.) was investigated using UHPLC-LTQ OrbiTrap MS.
The obtained findings revealed that the seed coats of the examined pea
genotypes possess a unique phenolic composition compared to previously
studied European cultivars. In total, 41 phenolic compounds have been
identified. The seed coats of the studied cultivars contained certain amounts
of rosmarinic acid, rutin, galangin, morin, naringin, hesperetin and
pinocembrin as well as ten flavonol glycosides that had not been reported
previously. Additionally, the total phenolic content, antioxidant activity
and metal chelating capacity of extracts was determined using
Folin-Ciocalteu’s method, 2,2-diphenyl-1-picrylhydrazyl radical scavenging
assay, ferric ion-reducing capacity and ferrous ion-chelating capacity assay,
respectively. Dark colored genotypes MBK 168 and MBK 173 possessed the
highest total phenolic contents as well the strongest antioxidant activities.
On the other hand, bright colored genotypes MBK 88 and MBK 90 exhibited the
strongest metal-chelating capacities. The examined pea seed coats may be
considered as important potential contributors to human health due to the
presence of bioactive phenolic constituents. In addition, our results could
be used as a guideline for breeding new pea cultivars with high antioxidant
activities applicable in the formulation of functional food products.
[Projekat Ministarstva nauke Republike Srbije, br. 173005 i br. 172017] |
|---|---|
| ISSN: | 0354-4664 1821-4339 |