Single molecule imaging of protein aggregation in Dementia: Methods, insights and prospects
The aggregation of misfolded proteins is a fundamental pathology in neurodegeneration which remains poorly understood due to its exceptional complexity and lack of appropriate characterization tools that can probe the role of the low concentrations of heterogeneous protein aggregates formed during t...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-06-01
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| Series: | Neurobiology of Disease |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0969996121000760 |
| _version_ | 1818948575397150720 |
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| author | John S.H. Danial David Klenerman |
| author_facet | John S.H. Danial David Klenerman |
| author_sort | John S.H. Danial |
| collection | DOAJ |
| description | The aggregation of misfolded proteins is a fundamental pathology in neurodegeneration which remains poorly understood due to its exceptional complexity and lack of appropriate characterization tools that can probe the role of the low concentrations of heterogeneous protein aggregates formed during the progression of the disease. In this review, we explain the principles underlying the operation of single molecule microscopy, an imaging method that can resolve molecules one-by-one, its application to imaging and characterizing individual protein aggregates in human samples and in vitro as well as the important questions in neurobiology this has answered and can answer. |
| first_indexed | 2024-12-20T08:48:59Z |
| format | Article |
| id | doaj.art-a783b152e17548cc8d0b30191f232658 |
| institution | Directory Open Access Journal |
| issn | 1095-953X |
| language | English |
| last_indexed | 2024-12-20T08:48:59Z |
| publishDate | 2021-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Neurobiology of Disease |
| spelling | doaj.art-a783b152e17548cc8d0b30191f2326582022-12-21T19:46:10ZengElsevierNeurobiology of Disease1095-953X2021-06-01153105327Single molecule imaging of protein aggregation in Dementia: Methods, insights and prospectsJohn S.H. Danial0David Klenerman1Yusuf Hamied Department of Chemistry, University of Cambridge, Cambridge, United Kingdom; UK Dementia Research Institute, Department of Clinical Neurosciences, University of Cambridge, Cambridge, United Kingdom; Corresponding authors at: Yusuf Hamied Department of Chemistry, University of Cambridge, Cambridge, United Kingdom.Yusuf Hamied Department of Chemistry, University of Cambridge, Cambridge, United Kingdom; UK Dementia Research Institute, Department of Clinical Neurosciences, University of Cambridge, Cambridge, United Kingdom; Corresponding authors at: Yusuf Hamied Department of Chemistry, University of Cambridge, Cambridge, United Kingdom.The aggregation of misfolded proteins is a fundamental pathology in neurodegeneration which remains poorly understood due to its exceptional complexity and lack of appropriate characterization tools that can probe the role of the low concentrations of heterogeneous protein aggregates formed during the progression of the disease. In this review, we explain the principles underlying the operation of single molecule microscopy, an imaging method that can resolve molecules one-by-one, its application to imaging and characterizing individual protein aggregates in human samples and in vitro as well as the important questions in neurobiology this has answered and can answer.http://www.sciencedirect.com/science/article/pii/S0969996121000760Single molecule microscopyProtein aggregation |
| spellingShingle | John S.H. Danial David Klenerman Single molecule imaging of protein aggregation in Dementia: Methods, insights and prospects Neurobiology of Disease Single molecule microscopy Protein aggregation |
| title | Single molecule imaging of protein aggregation in Dementia: Methods, insights and prospects |
| title_full | Single molecule imaging of protein aggregation in Dementia: Methods, insights and prospects |
| title_fullStr | Single molecule imaging of protein aggregation in Dementia: Methods, insights and prospects |
| title_full_unstemmed | Single molecule imaging of protein aggregation in Dementia: Methods, insights and prospects |
| title_short | Single molecule imaging of protein aggregation in Dementia: Methods, insights and prospects |
| title_sort | single molecule imaging of protein aggregation in dementia methods insights and prospects |
| topic | Single molecule microscopy Protein aggregation |
| url | http://www.sciencedirect.com/science/article/pii/S0969996121000760 |
| work_keys_str_mv | AT johnshdanial singlemoleculeimagingofproteinaggregationindementiamethodsinsightsandprospects AT davidklenerman singlemoleculeimagingofproteinaggregationindementiamethodsinsightsandprospects |