Isolation of <it>Plasmodium falciparum</it> by flow-cytometry: implications for single-trophozoite genotyping and parasite DNA purification for whole-genome high-throughput sequencing of archival samples

Isolation of <it>Plasmodium falciparum</it> by flow-cytometry: implications for single-trophozoite genotyping and parasite DNA purification for whole-genome high-throughput sequencing of archival samples

<p>Abstract</p> <p>Background</p> <p>Flow cytometry and cell sorting are powerful tools enabling the selection of particular cell types within heterogeneous cell mixtures. These techniques, combined with whole genome amplification that non-specifically amplify small amo...

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Bibliographic Details
Main Authors: Boissière Anne, Arnathau Céline, Duperray Christophe, Berry Laurence, Lachaud Laurence, Renaud François, Durand Patrick, Prugnolle Franck
Format: Article
Language:English
Published: BMC 2012-05-01
Series:Malaria Journal
Subjects:
<it>Plasmodium</it>
FACS
Cytometry
Genotyping
Whole genome sequencing
Online Access:http://www.malariajournal.com/content/11/1/163

Internet

http://www.malariajournal.com/content/11/1/163

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