Protocol for rapid and accurate quantification of phospholipids in yeast and mammalian systems using LC-MS

Protocol for rapid and accurate quantification of phospholipids in yeast and mammalian systems using LC-MS

Summary: We describe a protocol for measuring phospholipid class and fatty acid composition in the budding yeast Saccharomyces cerevisiae using a liquid chromatography-mass spectrometry (LC-MS)-based approach. We compile a mass spectral-retention time library verified for major phospholipids in the...

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Bibliographic Details
Main Authors: Sen Yang, Jingyuan Xue, Cunqi Ye
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:STAR Protocols
Subjects:
Cell biology
Metabolomics
Model organisms
Mass spectrometry
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722006499

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http://www.sciencedirect.com/science/article/pii/S2666166722006499

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