Ligand-induced endocytosis and nuclear localization of angiotensin II receptors expressed in CHO cells
A construct (AT1R-NF) containing a "Flag" sequence added to the N-terminus of the rat AT1 receptor was stably expressed in Chinese hamster ovary cells and quantified in the cell membrane by confocal microscopy after reaction with a fluorescein-labeled anti-Flag monoclonal antibody. Angiote...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2001-09-01
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| Series: | Brazilian Journal of Medical and Biological Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000900011 |
| _version_ | 1811191904527712256 |
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| author | A.J. Merjan C.A. Kanashiro J.E. Krieger S.W. Han A.C.M. Paiva |
| author_facet | A.J. Merjan C.A. Kanashiro J.E. Krieger S.W. Han A.C.M. Paiva |
| author_sort | A.J. Merjan |
| collection | DOAJ |
| description | A construct (AT1R-NF) containing a "Flag" sequence added to the N-terminus of the rat AT1 receptor was stably expressed in Chinese hamster ovary cells and quantified in the cell membrane by confocal microscopy after reaction with a fluorescein-labeled anti-Flag monoclonal antibody. Angiotensin II bound to AT1R-NF and induced endocytosis with a half-time of 2 min. After 60-90 min, fluorescence accumulated around the cell nucleus, suggesting migration of the ligand-receptor complex to the nuclear membrane. Angiotensin antagonists also induced endocytosis, suggesting that a common step in the transduction signal mechanism occurring after ligand binding may be responsible for the ligand-receptor complex internalization. |
| first_indexed | 2024-04-11T23:43:45Z |
| format | Article |
| id | doaj.art-a793831c1d4b4f7f9390b8f2e82b8261 |
| institution | Directory Open Access Journal |
| issn | 0100-879X 1414-431X |
| language | English |
| last_indexed | 2024-04-11T23:43:45Z |
| publishDate | 2001-09-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-a793831c1d4b4f7f9390b8f2e82b82612022-12-22T03:56:43ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X2001-09-013491175118310.1590/S0100-879X2001000900011Ligand-induced endocytosis and nuclear localization of angiotensin II receptors expressed in CHO cellsA.J. MerjanC.A. KanashiroJ.E. KriegerS.W. HanA.C.M. PaivaA construct (AT1R-NF) containing a "Flag" sequence added to the N-terminus of the rat AT1 receptor was stably expressed in Chinese hamster ovary cells and quantified in the cell membrane by confocal microscopy after reaction with a fluorescein-labeled anti-Flag monoclonal antibody. Angiotensin II bound to AT1R-NF and induced endocytosis with a half-time of 2 min. After 60-90 min, fluorescence accumulated around the cell nucleus, suggesting migration of the ligand-receptor complex to the nuclear membrane. Angiotensin antagonists also induced endocytosis, suggesting that a common step in the transduction signal mechanism occurring after ligand binding may be responsible for the ligand-receptor complex internalization.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000900011angiotensin IIagonist-induced receptor internalizationendocytosisfluorescence-tagged receptornuclear angiotensin receptorsrenin-angiotensin system |
| spellingShingle | A.J. Merjan C.A. Kanashiro J.E. Krieger S.W. Han A.C.M. Paiva Ligand-induced endocytosis and nuclear localization of angiotensin II receptors expressed in CHO cells Brazilian Journal of Medical and Biological Research angiotensin II agonist-induced receptor internalization endocytosis fluorescence-tagged receptor nuclear angiotensin receptors renin-angiotensin system |
| title | Ligand-induced endocytosis and nuclear localization of angiotensin II receptors expressed in CHO cells |
| title_full | Ligand-induced endocytosis and nuclear localization of angiotensin II receptors expressed in CHO cells |
| title_fullStr | Ligand-induced endocytosis and nuclear localization of angiotensin II receptors expressed in CHO cells |
| title_full_unstemmed | Ligand-induced endocytosis and nuclear localization of angiotensin II receptors expressed in CHO cells |
| title_short | Ligand-induced endocytosis and nuclear localization of angiotensin II receptors expressed in CHO cells |
| title_sort | ligand induced endocytosis and nuclear localization of angiotensin ii receptors expressed in cho cells |
| topic | angiotensin II agonist-induced receptor internalization endocytosis fluorescence-tagged receptor nuclear angiotensin receptors renin-angiotensin system |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000900011 |
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