Improvement of HEK293 Cell Growth by Adapting Hydrodynamic Stress and Predicting Cell Aggregate Size Distribution

Improvement of HEK293 Cell Growth by Adapting Hydrodynamic Stress and Predicting Cell Aggregate Size Distribution

HEK293 is a widely used cell line in the fields of research and industry. It is assumed that these cells are sensitive to hydrodynamic stress. The aim of this research was to use particle image velocimetry validated computational fluid dynamics (CFD) to determine the hydrodynamic stress in both shak...

Detaylı Bibliyografya
Asıl Yazarlar:	Stefan Seidel, Rüdiger W. Maschke, Fruhar Mozaffari, Regine Eibl-Schindler, Dieter Eibl
Materyal Türü:	Makale
Dil:	English
Baskı/Yayın Bilgisi:	MDPI AG 2023-04-01
Seri Bilgileri:	Bioengineering
Konular:	aggregate size distribution biochemical engineering CFD computational fluid dynamics energy dissipation rate fluid dynamic stress HEK293 suspension culture
Online Erişim:	https://www.mdpi.com/2306-5354/10/4/478

Benzer Materyaller

Nanotoxic Effects of Silver Nanoparticles on Normal HEK-293 Cells in Comparison to Cancerous HeLa Cell Line
Yazar:: Liu X, ve diğerleri
Baskı/Yayın Bilgisi: (2021-02-01)

Affecting HEK293 Cell Growth and Production Performance by Modifying the Expression of Specific Genes
Yazar:: Laura Abaandou, ve diğerleri
Baskı/Yayın Bilgisi: (2021-07-01)

The Lcn2-engineered HEK-293 cells show senescence under stressful condition
Yazar:: Bahareh Bahmani, ve diğerleri
Baskı/Yayın Bilgisi: (2015-05-01)

Evaluation of anticancer peptide VEGF111b secretion in HEK293 human cells
Yazar:: Morteza Sadeghi, ve diğerleri
Baskı/Yayın Bilgisi: (2017-04-01)

Tetracycline-mediated fluorescence correlates with passage number and β-galactosidase activity in HeLa and HEK293T cells
Yazar:: Chileab Redwood-Sawyerr, ve diğerleri
Baskı/Yayın Bilgisi: (2023-07-01)

Inducible HEK293 AAV packaging cell lines expressing Rep proteins
Yazar:: Lovro Jalšić, ve diğerleri
Baskı/Yayın Bilgisi: (2023-09-01)

Immortalized HEK 293 Kidney Cell Lines as Models of Renal Cells: Friends or Foes?
Yazar:: Cesare Achilli, ve diğerleri
Baskı/Yayın Bilgisi: (2018-08-01)

Screening for androgen agonists using autonomously bioluminescent HEK293 reporter cells
Yazar:: Tingting Xu, ve diğerleri
Baskı/Yayın Bilgisi: (2021-08-01)

The Effect of Aspirin and Ibuprofen on the Proliferation of Cervical Cancer Cells (HeLa) Compared to Non-Cancerous Cells (HEK 293) in Cell Culture Medium
Yazar:: Rahim Ahmadi, ve diğerleri
Baskı/Yayın Bilgisi: (2018-07-01)

HEK293 Cell Line as a Platform to Produce Recombinant Proteins and Viral Vectors
Yazar:: Evan Tan, ve diğerleri
Baskı/Yayın Bilgisi: (2021-12-01)

Transient Expression of a Recombinant Monoclonal Antibody in HEK293T Cells
Yazar:: Omid Mohammadian, ve diğerleri
Baskı/Yayın Bilgisi: (2018-09-01)

Construction and characterization of human embryonic kidney-(HEK)-293T cell overexpressing truncated α4 integrin
Yazar:: Azam Fatahi, ve diğerleri
Baskı/Yayın Bilgisi: (2018-01-01)

Enhancement of Transgene Expression by Mild Hypothermia Is Promoter Dependent in HEK293 Cells
Yazar:: Min Ho Jang, ve diğerleri
Baskı/Yayın Bilgisi: (2021-08-01)

Impact of lipopolysaccharides on cultivation and recombinant protein expression in human embryonal kidney (HEK‐293) cells
Yazar:: Christine Faust, ve diğerleri
Baskı/Yayın Bilgisi: (2021-11-01)

Knocking out Ornithine Decarboxylase Antizyme 1 (OAZ1) Improves Recombinant Protein Expression in the HEK293 Cell Line
Yazar:: Laura Abaandou, ve diğerleri
Baskı/Yayın Bilgisi: (2018-06-01)

Pharmacological mechanism of indigenous herb Exacum lawii on cisplatin instigated toxicity in human embryonic kidney cells (HEK-293)
Yazar:: Sonam Sharma
Baskı/Yayın Bilgisi: (2023-05-01)

Temporal insights into molecular and cellular responses during rAAV production in HEK293T cells
Yazar:: Alok Tanala Patra, ve diğerleri
Baskı/Yayın Bilgisi: (2024-09-01)

A human expression system based on HEK293 for the stable production of recombinant erythropoietin
Yazar:: Chin, Christine Lin, ve diğerleri
Baskı/Yayın Bilgisi: (2020)

Kinetics and Specificity of HEK293T Extracellular Vesicle Uptake using Imaging Flow Cytometry
Yazar:: Brian J. Jurgielewicz, ve diğerleri
Baskı/Yayın Bilgisi: (2020-08-01)

CFD modelling of a wave-mixed bioreactor with complex geometry and two degrees of freedom motion
Yazar:: Stefan Seidel, ve diğerleri
Baskı/Yayın Bilgisi: (2022-10-01)

In Vitro Toxicity Evaluation of Cyanotoxins Cylindrospermopsin and Microcystin-LR on Human Kidney HEK293 Cells
Yazar:: Leticia Diez-Quijada, ve diğerleri
Baskı/Yayın Bilgisi: (2022-06-01)

Evaluation of Iranian Snake ‘Macrovipera lebetina’ Venom Cytotoxicity in Kidney Cell Line HEK-293
Yazar:: Hourieh Esmaeili Jahromi, ve diğerleri
Baskı/Yayın Bilgisi: (2016-03-01)

Enhancing the Butyrylcholinesterase Activity in HEK-293 Cell Line by Dual-Promoter Vector Decorated on Lipofectamine
Yazar:: Mirzaie V, ve diğerleri
Baskı/Yayın Bilgisi: (2020-09-01)

Single nucleotide-level mapping of DNA double-strand breaks in human HEK293T cells
Yazar:: Bernard J. Pope, ve diğerleri
Baskı/Yayın Bilgisi: (2017-03-01)

Differential proteome analysis of human embryonic kidney cell line (HEK-293) following mycophenolic acid treatment
Yazar:: Rahman Hazir, ve diğerleri
Baskı/Yayın Bilgisi: (2011-09-01)

Evaluation of the protective effects of quercetin and gallic acid against oxidative toxicity in rat’s kidney and HEK-293 cells
Yazar:: Sunny O. Abarikwu, ve diğerleri
Baskı/Yayın Bilgisi: (2020-01-01)

COG Complex complexities: Detailed characterization of a complete set of HEK293T cells lacking individual COG subunits
Yazar:: Jessica eBailey Blackburn, ve diğerleri
Baskı/Yayın Bilgisi: (2016-03-01)

Enhancement effect of urea toward electroporation-mediated plasmid transfection efficiency in the HEK-293 cell line
Yazar:: Mahshid Mowla, ve diğerleri
Baskı/Yayın Bilgisi: (2024-12-01)

Trio-Drug Combination of Sodium Valproate, Baclofen and Thymoquinone Exhibits Synergistic Anticonvulsant Effects in Rats and Neuro-Protective Effects in HEK-293 Cells
Yazar:: Faheem Hyder Pottoo, ve diğerleri
Baskı/Yayın Bilgisi: (2022-09-01)

Distinct mechanical behavior of HEK293 cells in adherent and suspended states
Yazar:: Seyed Mohammad Ali Haghparast, ve diğerleri
Baskı/Yayın Bilgisi: (2015-07-01)

Expression of glycosylated human prolactin in HEK293 cells and related N-glycan composition analysis
Yazar:: Felipe D. Silva, ve diğerleri
Baskı/Yayın Bilgisi: (2019-08-01)

Beta-catenin Forms Protein Aggregation at High Concentrations in HEK293TCells
Yazar:: Marie Saghaeian Jazi, ve diğerleri
Baskı/Yayın Bilgisi: (2017-01-01)

Optimization of Culture Media and Feeding Strategy for High Titer Production of an Adenoviral Vector in HEK 293 Fed-Batch Culture
Yazar:: Chun Fang Shen, ve diğerleri
Baskı/Yayın Bilgisi: (2024-05-01)

Designing and evaluation of MERS-CoV siRNAs in HEK-293 cell line
Yazar:: Sayed Sartaj Sohrab, ve diğerleri
Baskı/Yayın Bilgisi: (2021-02-01)

Nephroprotective potential of Sharbat-e-Bazoori Motadil (sugar-free) in HEK-293 cells and Wistar rats against cisplatin induced nephrotoxicity
Yazar:: Mohammad Umar Khan, ve diğerleri
Baskı/Yayın Bilgisi: (2022-04-01)

Integral membrane protein expression of human CD25 on the cell surface of HEK293 cell line: the available cellular model of CD25 positive to facilitate in vitro developing assays
Yazar:: Dehbashi Moein, ve diğerleri
Baskı/Yayın Bilgisi: (2019-09-01)

Olive compounds attenuate oxidative damage induced in HEK-293 cells via MAPK signaling pathway
Yazar:: Amina Maalej, ve diğerleri
Baskı/Yayın Bilgisi: (2017-12-01)

Interaction of Styrylpyridinium Compound with Pathogenic <i>Candida albicans</i> Yeasts and Human Embryonic Kidney HEK-293 Cells
Yazar:: Simona Vaitkienė, ve diğerleri
Baskı/Yayın Bilgisi: (2020-12-01)

HEK293T Cells with TFAM Disruption by CRISPR-Cas9 as a Model for Mitochondrial Regulation
Yazar:: Vanessa Cristina de Oliveira, ve diğerleri
Baskı/Yayın Bilgisi: (2021-12-01)

Monomethyl auristatin E Exhibits Potent Cytotoxic Activity against Human Cancer Cell Lines SKBR3 and HEK293
Yazar:: Meghdad Abdollahpour-Alitappeh, ve diğerleri
Baskı/Yayın Bilgisi: (2017-10-01)