Protocol to characterize mouse intestinal epithelial cell lineage using Opal multiplex immunofluorescence

Protocol to characterize mouse intestinal epithelial cell lineage using Opal multiplex immunofluorescence

Summary: Applying Opal multiplex immunofluorescence (OMI) to characterize intestinal tissues of genetically engineered mouse models provides an excellent tool for studying complex processes. However, detecting appropriate signals from multiple target molecules is challenging. Here, we present a prot...

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Bibliographic Details
Main Authors: Anxo Martinez-Ordoñez, Hiroto Kinoshita, Tania Cid-Diaz, Angeles Duran, Marta Osrodek, Maria T. Diaz-Meco, Jorge Moscat
Format: Article
Language:English
Published: Elsevier 2024-09-01
Series:STAR Protocols
Subjects:
Cancer
Microscopy
Model Organisms
Molecular Biology
In Situ Hybridization
Molecular/Chemical Probes
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724004684

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http://www.sciencedirect.com/science/article/pii/S2666166724004684

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