Reactive Acrylamide-Modified DNA Traps for Accurate Cross-Linking with Cysteine Residues in DNA–Protein Complexes Using Mismatch Repair Protein MutS as a Model

Reactive Acrylamide-Modified DNA Traps for Accurate Cross-Linking with Cysteine Residues in DNA–Protein Complexes Using Mismatch Repair Protein MutS as a Model

Covalent protein capture (cross-linking) by reactive DNA derivatives makes it possible to investigate structural features by fixing complexes at different stages of DNA–protein recognition. The most common cross-linking methods are based on reactive groups that interact with native or engineered cys...

Full description

Bibliographic Details
Main Authors: Mayya V. Monakhova, Elena A. Kubareva, Kirill K. Kolesnikov, Viktor A. Anashkin, Egor M. Kosaretskiy, Maria I. Zvereva, Elena A. Romanova, Peter Friedhoff, Tatiana S. Oretskaya, Timofei S. Zatsepin
Format: Article
Language:English
Published: MDPI AG 2022-04-01
Series:Molecules
Subjects:
regioselectivity
DNA modification
DNA–protein complex
modified oligonucleotide
crosslinking
DNA mismatch repair
Online Access:https://www.mdpi.com/1420-3049/27/8/2438

Internet

https://www.mdpi.com/1420-3049/27/8/2438

Similar Items