Comparison of CRISPR and Marker-Based Methods for the Engineering of Phage T7
With the recent rise in interest in using lytic bacteriophages as therapeutic agents, there is an urgent requirement to understand their fundamental biology to enable the engineering of their genomes. Current methods of phage engineering rely on homologous recombination, followed by a system of sele...
Main Authors: | , , , , , , , , , |
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Format: | Article |
Language: | English |
Published: |
MDPI AG
2020-02-01
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Series: | Viruses |
Subjects: | |
Online Access: | https://www.mdpi.com/1999-4915/12/2/193 |