Meta-Surface Slide for High-Contrast Dark-Field Imaging
A label-free microscopy technology, dark-field microscopy, is widely used for providing high-contrast imaging for weakly scattering materials and unstained samples. However, traditional dark-field microscopes often require additional components and larger condensers as the numerical aperture increas...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Photonics |
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| Online Access: | https://www.mdpi.com/2304-6732/10/7/775 |
| _version_ | 1827732046430076928 |
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| author | Jianan Shao Ruiyi Chen Dehua Zhu Yu Cao Wenwen Liu Wei Xue |
| author_facet | Jianan Shao Ruiyi Chen Dehua Zhu Yu Cao Wenwen Liu Wei Xue |
| author_sort | Jianan Shao |
| collection | DOAJ |
| description | A label-free microscopy technology, dark-field microscopy, is widely used for providing high-contrast imaging for weakly scattering materials and unstained samples. However, traditional dark-field microscopes often require additional components and larger condensers as the numerical aperture increases. A solution to this is the use of a meta-surface slide. This slide utilizes a multilayer meta-surface and quantum dots to convert incident white light into a red glow cone emitted at a larger angle. This enables the slide to be used directly with conventional biological microscopy to achieve dark-field imaging. This paper focuses on the design and preparation of the meta-surface and demonstrates that using the meta-surface in a standard transmission optical microscope results in a dark-field image with higher contrast than a bright-field image, especially when observing samples with micron-sized structures. |
| first_indexed | 2024-03-11T00:44:10Z |
| format | Article |
| id | doaj.art-a856ddebe6f24368a8537ac568f668ff |
| institution | Directory Open Access Journal |
| issn | 2304-6732 |
| language | English |
| last_indexed | 2024-03-11T00:44:10Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Photonics |
| spelling | doaj.art-a856ddebe6f24368a8537ac568f668ff2023-11-18T20:57:58ZengMDPI AGPhotonics2304-67322023-07-0110777510.3390/photonics10070775Meta-Surface Slide for High-Contrast Dark-Field ImagingJianan Shao0Ruiyi Chen1Dehua Zhu2Yu Cao3Wenwen Liu4Wei Xue5Zhejiang Provincial Key Laboratory of Laser Processing Robotics, College of Mechanical and Electrical Engineering, Wenzhou University, Wenzhou 325035, ChinaZhejiang Provincial Key Laboratory of Laser Processing Robotics, College of Mechanical and Electrical Engineering, Wenzhou University, Wenzhou 325035, ChinaZhejiang Provincial Key Laboratory of Laser Processing Robotics, College of Mechanical and Electrical Engineering, Wenzhou University, Wenzhou 325035, ChinaZhejiang Provincial Key Laboratory of Laser Processing Robotics, College of Mechanical and Electrical Engineering, Wenzhou University, Wenzhou 325035, ChinaZhejiang Provincial Key Laboratory of Laser Processing Robotics, College of Mechanical and Electrical Engineering, Wenzhou University, Wenzhou 325035, ChinaZhejiang Provincial Key Laboratory of Laser Processing Robotics, College of Mechanical and Electrical Engineering, Wenzhou University, Wenzhou 325035, ChinaA label-free microscopy technology, dark-field microscopy, is widely used for providing high-contrast imaging for weakly scattering materials and unstained samples. However, traditional dark-field microscopes often require additional components and larger condensers as the numerical aperture increases. A solution to this is the use of a meta-surface slide. This slide utilizes a multilayer meta-surface and quantum dots to convert incident white light into a red glow cone emitted at a larger angle. This enables the slide to be used directly with conventional biological microscopy to achieve dark-field imaging. This paper focuses on the design and preparation of the meta-surface and demonstrates that using the meta-surface in a standard transmission optical microscope results in a dark-field image with higher contrast than a bright-field image, especially when observing samples with micron-sized structures.https://www.mdpi.com/2304-6732/10/7/775dark-field microscopyhigh-contrast imagingmeta-surfacequantum dots |
| spellingShingle | Jianan Shao Ruiyi Chen Dehua Zhu Yu Cao Wenwen Liu Wei Xue Meta-Surface Slide for High-Contrast Dark-Field Imaging Photonics dark-field microscopy high-contrast imaging meta-surface quantum dots |
| title | Meta-Surface Slide for High-Contrast Dark-Field Imaging |
| title_full | Meta-Surface Slide for High-Contrast Dark-Field Imaging |
| title_fullStr | Meta-Surface Slide for High-Contrast Dark-Field Imaging |
| title_full_unstemmed | Meta-Surface Slide for High-Contrast Dark-Field Imaging |
| title_short | Meta-Surface Slide for High-Contrast Dark-Field Imaging |
| title_sort | meta surface slide for high contrast dark field imaging |
| topic | dark-field microscopy high-contrast imaging meta-surface quantum dots |
| url | https://www.mdpi.com/2304-6732/10/7/775 |
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