Effect of Exogenous Neutral Protease Fermentation on Cigar Leaf Quality
In order to reveal the effect of exogenous neutral protease addition on the quality of cigar tobacco leaves during fermentation. Taking Sichuan Shifang cigar tobacco leaves as the research object, the changes of conventional chemical composition, amino acid content, organic acids and other substance...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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EDP Sciences
2023-01-01
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| Series: | BIO Web of Conferences |
| Online Access: | https://www.bio-conferences.org/articles/bioconf/pdf/2023/05/bioconf_bfs2023_01020.pdf |
| _version_ | 1797808081708515328 |
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| author | Zhang Wei Yang Xin’an Zhang Qi Zhu Wenkui Lu Yan Shang Xiaoli |
| author_facet | Zhang Wei Yang Xin’an Zhang Qi Zhu Wenkui Lu Yan Shang Xiaoli |
| author_sort | Zhang Wei |
| collection | DOAJ |
| description | In order to reveal the effect of exogenous neutral protease addition on the quality of cigar tobacco leaves during fermentation. Taking Sichuan Shifang cigar tobacco leaves as the research object, the changes of conventional chemical composition, amino acid content, organic acids and other substances in tobacco leaves after natural fermentation and fermentation with exogenous enzyme preparations were investigated, and the changes of sensory quality of cigar tobacco leaves before and after fermentation were also investigated. The results show that: 1) After fermentation, the content of total sugar, reducing sugar, total nitrogen, chlorogenic acid and rutin in tobacco leaves decreased, and the content of reducing sugar fermented with enzyme preparation decreased more. 2) After fermentation, the total amount of free amino acids in tobacco leaves increased. With the increase of exogenous neutral protease, the content of amino acids first increased and then decreased. 3) After fermentation, the content of organic acids in tobacco leaves increased. When the amount of enzyme added was 2.5g/kg leaves, the increase of organic acid content was greater than that of natural fermentation, and the other additions of enzyme were close to that of natural fermentation. 4) After fermentation, the content of plastid pigment in tobacco leaves decreased slightly. With the increase of neutral protease, the content of plastid pigment increased. 5) After the fermentation of exogenous enzyme preparation, the sensory quality of tobacco leaves is improved, but too much protease will produce bitter taste. |
| first_indexed | 2024-03-13T06:32:10Z |
| format | Article |
| id | doaj.art-a866c2f1d623495fa1a01f4b31dc255b |
| institution | Directory Open Access Journal |
| issn | 2117-4458 |
| language | English |
| last_indexed | 2024-03-13T06:32:10Z |
| publishDate | 2023-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | BIO Web of Conferences |
| spelling | doaj.art-a866c2f1d623495fa1a01f4b31dc255b2023-06-09T08:44:26ZengEDP SciencesBIO Web of Conferences2117-44582023-01-01600102010.1051/bioconf/20236001020bioconf_bfs2023_01020Effect of Exogenous Neutral Protease Fermentation on Cigar Leaf QualityZhang Wei0Yang Xin’an1Zhang Qi2Zhu Wenkui3Lu Yan4Shang Xiaoli5Sichuan China Tobacco Industry Co., LtdZhengzhou Tobacco Research Institute of CNTCZhengzhou Tobacco Research Institute of CNTCZhengzhou Tobacco Research Institute of CNTCZhengzhou Tobacco Research Institute of CNTCZhengzhou Tobacco Research Institute of CNTCIn order to reveal the effect of exogenous neutral protease addition on the quality of cigar tobacco leaves during fermentation. Taking Sichuan Shifang cigar tobacco leaves as the research object, the changes of conventional chemical composition, amino acid content, organic acids and other substances in tobacco leaves after natural fermentation and fermentation with exogenous enzyme preparations were investigated, and the changes of sensory quality of cigar tobacco leaves before and after fermentation were also investigated. The results show that: 1) After fermentation, the content of total sugar, reducing sugar, total nitrogen, chlorogenic acid and rutin in tobacco leaves decreased, and the content of reducing sugar fermented with enzyme preparation decreased more. 2) After fermentation, the total amount of free amino acids in tobacco leaves increased. With the increase of exogenous neutral protease, the content of amino acids first increased and then decreased. 3) After fermentation, the content of organic acids in tobacco leaves increased. When the amount of enzyme added was 2.5g/kg leaves, the increase of organic acid content was greater than that of natural fermentation, and the other additions of enzyme were close to that of natural fermentation. 4) After fermentation, the content of plastid pigment in tobacco leaves decreased slightly. With the increase of neutral protease, the content of plastid pigment increased. 5) After the fermentation of exogenous enzyme preparation, the sensory quality of tobacco leaves is improved, but too much protease will produce bitter taste.https://www.bio-conferences.org/articles/bioconf/pdf/2023/05/bioconf_bfs2023_01020.pdf |
| spellingShingle | Zhang Wei Yang Xin’an Zhang Qi Zhu Wenkui Lu Yan Shang Xiaoli Effect of Exogenous Neutral Protease Fermentation on Cigar Leaf Quality BIO Web of Conferences |
| title | Effect of Exogenous Neutral Protease Fermentation on Cigar Leaf Quality |
| title_full | Effect of Exogenous Neutral Protease Fermentation on Cigar Leaf Quality |
| title_fullStr | Effect of Exogenous Neutral Protease Fermentation on Cigar Leaf Quality |
| title_full_unstemmed | Effect of Exogenous Neutral Protease Fermentation on Cigar Leaf Quality |
| title_short | Effect of Exogenous Neutral Protease Fermentation on Cigar Leaf Quality |
| title_sort | effect of exogenous neutral protease fermentation on cigar leaf quality |
| url | https://www.bio-conferences.org/articles/bioconf/pdf/2023/05/bioconf_bfs2023_01020.pdf |
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