A Long-Chain Dextran Produced by <i>Weissella cibaria</i> Boosts the Diversity of Health-Related Gut Microbes Ex Vivo
Long-chain dextrans are α-glucans that can be produced by lactic acid bacteria. NextDext<sup>TM</sup>, a specific long-chain dextran with a high degree of polymerisation, produced using <i>Weissella cibaria</i>, was recently shown to exert prebiotic potential in vitro. In thi...
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MDPI AG
2024-01-01
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| Online Access: | https://www.mdpi.com/2079-7737/13/1/51 |
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| author | Maria Tintoré Jordi Cuñé Lam Dai Vu Jonas Poppe Pieter Van den Abbeele Aurélien Baudot Carlos de Lecea |
| author_facet | Maria Tintoré Jordi Cuñé Lam Dai Vu Jonas Poppe Pieter Van den Abbeele Aurélien Baudot Carlos de Lecea |
| author_sort | Maria Tintoré |
| collection | DOAJ |
| description | Long-chain dextrans are α-glucans that can be produced by lactic acid bacteria. NextDext<sup>TM</sup>, a specific long-chain dextran with a high degree of polymerisation, produced using <i>Weissella cibaria</i>, was recently shown to exert prebiotic potential in vitro. In this study, the ex vivo SIFR<sup>®</sup> technology, recently validated to provide predictive insights into gut microbiome modulation down to the species level, was used to investigate the effects of this long-chain dextran on the gut microbiota of six human adults that altogether covered different enterotypes. A novel community modulation score (CMS) was introduced based on the strength of quantitative 16S rRNA gene sequencing and the highly controlled ex vivo conditions. This CMS overcomes the limitations of traditional α-diversity indices and its application in the current study revealed that dextran is a potent booster of microbial diversity compared to the reference prebiotic inulin (IN). Long-chain dextran not only exerted bifidogenic effects but also consistently promoted <i>Bacteroides</i> spp., <i>Parabacteroides distasonis</i> and butyrate-producing species like <i>Faecalibacterium prausnitzii</i> and <i>Anaerobutyricum hallii</i>. Further, long-chain dextran treatment resulted in lower gas production compared to IN, suggesting that long-chain dextran could be better tolerated. The additional increase in <i>Bacteroides</i> for dextran compared to IN is likely related to the higher propionate:acetate ratio, attributing potential to long-chain dextran for improving metabolic health and weight management. Moreover, the stimulation of butyrate by dextran suggests its potential for improving gut barrier function and inflammation. Overall, this study provides a novel tool for assessing gut microbial diversity ex vivo and positions long-chain dextran as a substrate that has unique microbial diversity enhancing properties. |
| first_indexed | 2024-03-08T11:04:36Z |
| format | Article |
| id | doaj.art-a899b7a1eca2412f9ccc2eace5b3b207 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-08T11:04:36Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-a899b7a1eca2412f9ccc2eace5b3b2072024-01-26T15:07:12ZengMDPI AGBiology2079-77372024-01-011315110.3390/biology13010051A Long-Chain Dextran Produced by <i>Weissella cibaria</i> Boosts the Diversity of Health-Related Gut Microbes Ex VivoMaria Tintoré0Jordi Cuñé1Lam Dai Vu2Jonas Poppe3Pieter Van den Abbeele4Aurélien Baudot5Carlos de Lecea6AB Biotek Human Nutrition and Health, Peterborough PE7 8QJ, UKAB Biotek Human Nutrition and Health, Peterborough PE7 8QJ, UKCryptobiotix SA, Technologiepark-Zwijnaarde 82, 9052 Ghent, BelgiumCryptobiotix SA, Technologiepark-Zwijnaarde 82, 9052 Ghent, BelgiumCryptobiotix SA, Technologiepark-Zwijnaarde 82, 9052 Ghent, BelgiumCryptobiotix SA, Technologiepark-Zwijnaarde 82, 9052 Ghent, BelgiumAB Biotek Human Nutrition and Health, Peterborough PE7 8QJ, UKLong-chain dextrans are α-glucans that can be produced by lactic acid bacteria. NextDext<sup>TM</sup>, a specific long-chain dextran with a high degree of polymerisation, produced using <i>Weissella cibaria</i>, was recently shown to exert prebiotic potential in vitro. In this study, the ex vivo SIFR<sup>®</sup> technology, recently validated to provide predictive insights into gut microbiome modulation down to the species level, was used to investigate the effects of this long-chain dextran on the gut microbiota of six human adults that altogether covered different enterotypes. A novel community modulation score (CMS) was introduced based on the strength of quantitative 16S rRNA gene sequencing and the highly controlled ex vivo conditions. This CMS overcomes the limitations of traditional α-diversity indices and its application in the current study revealed that dextran is a potent booster of microbial diversity compared to the reference prebiotic inulin (IN). Long-chain dextran not only exerted bifidogenic effects but also consistently promoted <i>Bacteroides</i> spp., <i>Parabacteroides distasonis</i> and butyrate-producing species like <i>Faecalibacterium prausnitzii</i> and <i>Anaerobutyricum hallii</i>. Further, long-chain dextran treatment resulted in lower gas production compared to IN, suggesting that long-chain dextran could be better tolerated. The additional increase in <i>Bacteroides</i> for dextran compared to IN is likely related to the higher propionate:acetate ratio, attributing potential to long-chain dextran for improving metabolic health and weight management. Moreover, the stimulation of butyrate by dextran suggests its potential for improving gut barrier function and inflammation. Overall, this study provides a novel tool for assessing gut microbial diversity ex vivo and positions long-chain dextran as a substrate that has unique microbial diversity enhancing properties.https://www.mdpi.com/2079-7737/13/1/51prebioticlong-chain dextranex vivo<i>Bifidobacterium</i><i>Bacteroides</i>propionate |
| spellingShingle | Maria Tintoré Jordi Cuñé Lam Dai Vu Jonas Poppe Pieter Van den Abbeele Aurélien Baudot Carlos de Lecea A Long-Chain Dextran Produced by <i>Weissella cibaria</i> Boosts the Diversity of Health-Related Gut Microbes Ex Vivo Biology prebiotic long-chain dextran ex vivo <i>Bifidobacterium</i> <i>Bacteroides</i> propionate |
| title | A Long-Chain Dextran Produced by <i>Weissella cibaria</i> Boosts the Diversity of Health-Related Gut Microbes Ex Vivo |
| title_full | A Long-Chain Dextran Produced by <i>Weissella cibaria</i> Boosts the Diversity of Health-Related Gut Microbes Ex Vivo |
| title_fullStr | A Long-Chain Dextran Produced by <i>Weissella cibaria</i> Boosts the Diversity of Health-Related Gut Microbes Ex Vivo |
| title_full_unstemmed | A Long-Chain Dextran Produced by <i>Weissella cibaria</i> Boosts the Diversity of Health-Related Gut Microbes Ex Vivo |
| title_short | A Long-Chain Dextran Produced by <i>Weissella cibaria</i> Boosts the Diversity of Health-Related Gut Microbes Ex Vivo |
| title_sort | long chain dextran produced by i weissella cibaria i boosts the diversity of health related gut microbes ex vivo |
| topic | prebiotic long-chain dextran ex vivo <i>Bifidobacterium</i> <i>Bacteroides</i> propionate |
| url | https://www.mdpi.com/2079-7737/13/1/51 |
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